生物学杂志 ›› 2020, Vol. 37 ›› Issue (5): 35-.doi: 10.3969/j.issn.2095-1736.2020.05.035

• 研究报告 • 上一篇    下一篇

果蝇tap基因表达模式的测定#br#

  

  1. 河南科技大学 医学院, 洛阳 471003
  • 出版日期:2020-10-18 发布日期:2020-10-14
  • 通讯作者: 胡澍,博士,副教授,研究方向为果蝇神经系统的发育学,E-mail:hushu51@sina.com
  • 作者简介:王越,硕士研究生,研究方向为果蝇神经系统的发育学,E-mail:343866456@qq.com
  • 基金资助:
    国家自然科学基金(No:31171256)

Test of expression pattern of tap gene in Drosophila melanoganster

  1. Medical College, Henan University of Science and Technology, Luoyang 471003, China
  • Online:2020-10-18 Published:2020-10-14

摘要: 果蝇tap基因因具有和哺乳动物ngn基因极高的碱基序列相似度,被定性为ngn同源基因。近二十年来,在已知ngn同源基因中,除tap以外,其他基因的功能和调控基本都得到了比较充分的研究,而对tap基因的相应研究几乎停滞。直到利用同源重组技术,将tap基因的编码序列准确地替换成Gal4蛋白的编码序列,培育出tapGal4品系,并与UAS-GFP品系杂交,通过对子代果蝇中GFP进行荧光染色,明确了tap基因在蕈形体发育过程的表达模式。进而通过量化分析,确定了tap基因在蕈形体发育中不具有原神经功能,而是对神经轴突的生长和靶向延伸起重要作用。主要展示了tap基因在大脑,复眼发育过程中的表达模式,利于针对性地在这些器官中敲除、敲低或过量表达Tap蛋白,进而根据由此产生的表型改变,探究tap基因在这些器官发育中的功能和调控。

关键词: 果蝇, tap基因表达模式, Gal4系统, 免疫荧光染色

Abstract: The tap gene of Drosophila melanogaster is characterized as ngn homologous gene because of its high base sequence similarity with mammal ngn gene. In the past two decades, the function and regulation of other genes except tap have been fully studied in the known ngn homologous group, while the corresponding research on tap gene has almost stagnated. Until used homologous recombination technique, the coding sequence of tap gene was accurately replaced with the coding sequence of Gal4 gene, and the tapGal4 strain was bred and cross with UAS-GFP strain. By fluorescence staining of GFP in Drosophila melanogaster, the expression pattern of tap gene in the development of the mushroom body was clearly observed. Furthermore, through quantitative analysis, it was determined that tap gene did not have proneural function in the development of mushroom body, but played an important role in the axon growth and right targeting. This paper mainly showed the expression pattern of tap gene in the development of brain and compound eye, which was helpful to knockout, knockdown or overexpress Tap protein in these organs, and then explore the function and regulation of tap gene in the development of these organs according to the resulting phenotypic changes.

Key words: Drosophila melanoganster, expression pattern of tap gene, Gal4 system, immunofluorescence staining

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