生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (2): 24-.doi: 10.3969/j.issn.2095-1736.2026.02.024

• 研究报告 • 上一篇    下一篇

香雪兰幼苗热胁迫转录组初步分析

张　琰1， 李　勃2   

  1. 1. 山西药科职业学院制药系， 太原 030031； 2. 陕西省生物农业研究所， 西安 710043
  • 出版日期:2026-04-18 发布日期:2026-04-23
  • 通讯作者: 李勃，研究员，研究方向为植物病原菌及生物防治，E-mail：libo@xab.ac.cn
  • 作者简介:张琰，硕士，讲师，研究方向为植物生物化学与分子生物学，E-mail：zhangyan20032192@163.com
  • 基金资助:
    陕西省重点研发计划（一般项目）基金项目（2023-YBNY-263）

Preliminary transcriptome analysis of Freesia hybrida seedlings under high temperature stress

ZHANG Yan1, LI Bo2   

  1. 1. Pharmaceutical Department of Shanxi Pharmaceutical Vocational College, Taiyuan 030031, China;
    2. Shaanxi Institute of Bioagriculture, Xi’an 710043, China
  • Online:2026-04-18 Published:2026-04-23

摘要： 为解析香雪兰高温胁迫响应分子机制及推进耐热种质创新，以香雪兰‘Red River’为试验材料，对其进行不同时间（3、6、9和12 h）高温（40 ℃）处理后测定相应理化指标，利用RNA-seq对高温胁迫处理组（40 ℃、12 h）和对照组（20 ℃、12 h）的香雪兰幼苗进行高通量测序，筛选差异表达基因，并进行GO、KEGG分析。与对照组相比，40 ℃高温处理12 h后共鉴定到2961个差异表达基因，其中，1306个上调基因，1655个下调基因。GO分析显示，显著差异上调基因参与热响应、蛋白质折叠、蛋白质复合体寡聚化、对活性氧的响应、蛋白质进入线粒体基质等过程。KEGG分析显示，富集排名前面的代谢通路分别为内质网中蛋白质处理、植物激素信号转导、苯丙烷类生物合成、黄酮类化合物生物合成、光合作用—天线蛋白等。并且差异基因表达信息与高温胁迫下生理指标变化相一致。17个热激蛋白基因（共59个）、4个HSF转录因子（共23个）、CUL3A基因的表达水平在受到高温胁迫时显著升高，bZIP44、ERF63、ERF51、NAC42转录因子等协同构成香雪兰的耐热基因调控网络。植物激素信号转导通路中的18个差异基因涉及多种植物激素的调控，以应对高温胁迫的影响。

关键词: 香雪兰, 转录组分析, 高温胁迫, 生理指标

Abstract: The aim of this study was to elucidate the molecular mechanisms ofFreesia hybridain responding to high-temperature stress and to promote the innovation of heat-tolerant germplasm,F. hybrida‘Red River’ was used as the experimental material. Physiological parameters were measured under heat stress at 40 ℃ for varying durations (3, 6, 9 and 12 hours). RNA-seq was conducted on seedlings exposed to 40 ℃ for 12 hours (stress group) compared to those maintained at 20 ℃ for 12 hours (control group). Differentially expressed genes (DEGs) were then identified and analyzed using GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment. Compared to that of the control group, a total of 2961 DEGs were identified after 12 hours of treatment at 40 ℃, including 1306 up-regulated and 1655 down-regulated genes. The GO analysis revealed that the significantly upregulated DEGs were primarily involved in response to heat, protein folding, protein complex oligomerization, response to reactive oxygen species (ROS), and protein import into the mitochondrial matrix. The KEGG pathway enrichment analysis identified several top-ranked metabolic pathways, such as protein processing in the endoplasmic reticulum, plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis, and photosynthesis-antenna proteins. Notably, the DEGs were consistent with the observed physiological changes under high-temperature stress. Specifically, 17 heat shock protein (Hsp) genes (out of 59 identified), 4 heat shock transcription factors (HSF) (out of 23), and the CUL3A gene exhibited significant up-regulation during thermal stress. Furthermore, transcription factors such as bZIP44, ERF63, ERF51, and NAC42 collectively formed a thermotolerance regulatory network inF. hybrida. Within the plant hormone signal transduction pathway, 18 DEGs were identified as being involved in the regulation of various phytohormones in response to high-temperature stress.

Key words: Freesia hybrida, transcriptome analysis, high temperature stress, physiological indicators

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