生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (6): 56-.doi: 10.3969/j.issn.2095-1736.2024.06.056

• 研究报告 • 上一篇    下一篇

水分胁迫下德兰臭草对内生真菌感染的生理响应

张梦梦， 王传哲， 何嘉坤， 施　宠   

  1. 新疆农业大学 资源与环境学院， 乌鲁木齐 830052
  • 出版日期:2024-12-18 发布日期:2024-12-16
  • 通讯作者: 施宠，副教授，研究方向为禾草-内生真菌共生体抗逆性，E-mail:shichong98@163.com
  • 作者简介:张梦梦，硕士研究生，研究方向为禾草-内生真菌，E-mail:zhangmm328@163.com
  • 基金资助:
    国家自然科学基金地区基金项目(31760704)

Physiological responses of Melica transsilvanica to endophytic fungi infection under conditions of water stress

ZHANG Mengmeng, WANG Chuanzhe, HE Jiakun, SHI Chong   

  1. College of Resource and Environment, Xinjiang Agricultural University, Urumqi 830052, China
  • Online:2024-12-18 Published:2024-12-16

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摘要： 为探究不同水分胁迫下德兰臭草对内生真菌感染生理指标的影响，以内生真菌侵染德兰臭草植株（E+）与未侵染植株（E－）为材料进行盆栽控水试验，以土壤含水量60%～65%FWC为对照（CK），并设置土壤含水量85%～90%FWC为高湿处理（W）、15%～20%FWC为干旱处理（D），研究高湿与干旱处理下E+植株与E－植株生理指标的差异。结果表明：在胁迫7 d时，E+植株与E－植株的Pro含量、SOD活性在高湿与干旱条件下均有显著性差异（P＜0.05）；在胁迫14 d时，E+植株与E－植株的叶绿素含量、Pro含量、MDA含量、SOD活性、POD活性、APX活性和CAT活性在高湿和干旱条件下均有显著性差异（P＜0.05）；在胁迫21 d时，E+植株的MDA含量、细胞膜相对透性在高湿与干旱条件下均显著高于E－植株（P＜0.05）。内生真菌感染可以提高德兰臭草在水分胁迫下的抗性。

关键词: 水分胁迫, 内生真菌, 德兰臭草, 生理指标, 保护酶

Abstract: This study explored the impact of water stress on the physiological responses ofMelica transsilvanicato endophytic fungal infection. Using both endophyte-infected (E+) and non-infected (E－) plants, a controlled water experiment with soil moisture levels of 60%－65% field water capacity (FWC) was set up for the control (CK), 85%－90% FWC for the high humidity treatment (W), and 15%－20% FWC for the drought treatment (D). The significant differences were found in proline content and superoxide dismutase (SOD) activity between E+ and E－ plants following 7 days of both high humidity and drought stress (P<0.05). After 14 days of stress, the significant differences were observed in chlorophyll, proline, malondialdehyde (MDA) content, SOD, peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT) activities in E+ and E－ plants under the same conditions (P<0.05). After 21 days of stress, E+ plants had significantly increased MDA content and relative cell membrane permeability compared to E－ plants under both conditions (P<0.05). The results suggested that endophytic fungal infection enhanced the tolerance ofMelica transsilvanicato water stress.

Key words: water stress, endophytic fungi;Melica transsilvanica, physiological parameters, protective enzymes

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