生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (6): 62-.doi: 10.3969/j.issn.2095-1736.2024.06.062

• 研究报告 • 上一篇    下一篇

KXF6501特基拉芽孢杆菌发酵液处理采后蓝莓果实的代谢组分析

杨　阔1， 刘　地1， 伍建榕1，2， 邓　佳1,3， 王　芳1,2   

  1. 1. 西南林业大学 林学院， 昆明 650224； 2. 西南地区生物多样性保育国家林业和草原局重点实验室，
    昆明 650224； 3. 西南山地森林资源保育与利用教育部重点实验室， 昆明 650224
  • 出版日期:2024-12-18 发布日期:2024-12-16
  • 通讯作者: 王芳，副教授，主要从事资源微生物筛选与利用研究，E-mail:35445230@qq.com
  • 作者简介:杨阔，硕士研究生，研究方向为森林培育，E-mail:1941398927@qq.com
  • 基金资助:
    国家自然科学基金项目（ 32160395）；云南省“兴滇英才支持计划”青年人才项目（XDYC-QNRC-2022-0226）；云南省农业联合专项面上项目（202301BD070001-102）

Metabolomic analysis of postharvest blueberry fruits treated by Bacillus tequilensis KXF6501 fermentation

YANG Kuo1, LIU Di1, WU Jianrong1,2, DENG Jia1,3, WANG Fang1,2   

  1. 1. College of Forestry, Southwest Forestry University, Kunming 650224, China; 2. Key Laboratory of National
    Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224,
    China; 3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,
    Ministry of Education, Southwest Forestry University, Kunming 650224, China
  • Online:2024-12-18 Published:2024-12-16

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摘要： 探究采后蓝莓果实对特基拉芽孢杆菌发酵液处理后的响应机制，明确蓝莓果实内的代谢物种类与代谢途径变化，为特基拉芽孢杆菌发酵液处理提高蓝莓果实抗病机制提供理论依据。以无菌水浸泡处理为对照组，芽孢杆菌发酵液浸泡处理为试验组，开展果实抗灰霉病试验并利用非靶向代谢组分析蓝莓果实代谢物差异，在此基础上进一步测定果实的总酚和类黄酮含量。结果表明，特基拉芽孢杆菌发酵液处理后果实病斑直径显著降低，差异代谢物质共注释到77条代谢通路，富集相关性最高的20条代谢通路中共富集27种显著差异代谢物，有显著影响的6条通路分别为苯丙烷类化合物的生物合成、黄酮和黄酮醇生物合成、铁载体基团非核糖体肽的生物合成、类黄酮生物合成、细胞凋亡和C5-支链二元酸代谢，且蓝莓果实中的总酚类黄酮含量高于对照组处理，与代谢组分析结果一致。蓝莓果实中次生代谢物质的积累，提高了蓝莓果实的抗病能力。

关键词: 特基拉芽孢杆菌, 蓝莓果实, 代谢组, 次生代谢物

Abstract: This experiment was implemented to investigate the differences of the metabolites in blueberry fruits after the application ofBacillus tequilensisfermentation. The resistance mechanism of postharvest blueberry fruits treated byB.tequilensisfermentation was explored and the theoretical references for the application ofB.tequilensisin blueberry fruits storage and preservation were provided. Blueberry fruits were treated withB.tequilensisfermentation, the lesion diameters were measured. Subsequently, non-targeted metabolomics techniques were used to analyze the metabolites of blueberry fruits treated withB.tequilensisfermentation. Then, the content of phenolic compounds and flavonoids in blueberry fruit was determined. The result showed that blueberry fruit lesion diameters were significantly reduced after treated withB.tequilensisfermentation. Based on non-targeted metabolomics techniques, 77 pathways were annotated, and a total of 27 significant differential metabolites were enriched in the 20 metabolic pathways with the highest correlation in the group after treated withB.tequilensisfermentation. Among them, six pathways with significant influence were listed as follows: biosynthesis of phenylpropanoids, flavone and flavonol biosynthesis, biosynthesis of siderophore group nonribosomal peptides, flavonoid biosynthesis, apoptosis and C5-Branched dibasic acid metabolism. And the content of phenolic compounds and flavonoids in blueberry fruit was higher than that in the control treatment, which was consistent with the results of metabolomics analysis. After the treated withB.tequilensisfermentation, secondary metabolites accumulated in blueberry fruit, which in turn increased the disease resistance of blueberry fruits.

Key words: Bacillus tequilensis, blueberry fruit, metabolomics, secondary metabolites

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