生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (5): 61-.doi: 10.3969/j.issn.2095-1736.2024.05.061

• 研究报告 • 上一篇    下一篇

不同种植年限薰衣草根系分泌物研究

师仁增， 邓　霞， 王君竹， 柳雯雯， 李桂花， 郭子轩， 焦子伟   

  1. 伊犁师范大学 生物科学与技术学院， 伊宁 835000
  • 出版日期:2024-10-18 发布日期:2024-10-14
  • 通讯作者: 焦子伟，博士，教授，研究方向为根际微生态，E-mail:741285332@qq.com；郭子轩，硕士，助教，研究方向为根际微生态，E-mail:1418378004@qq.com；焦子伟和郭子轩为共同通信作者
  • 作者简介:师仁增，硕士研究生，研究方向为植物-根际微生物根际互作，E-mail:srenzeng@163.com；邓霞，硕士研究生，研究方向为植物-根际微生物互作，E-mail:dengxiayj@163.com；师仁增和邓霞为共同第一作者
  • 基金资助:
    国家自然科学基金项目（32160312）； 新疆维吾尔自治区重点研发项目（2022B02021-2）； 学实高层次人才岗位项目（YLSDXSDTR22001）

Study and analysis of root exudates of Lavandula angustifolia at different cultivation years

SHI Renzeng, DENG Xia, WANG Junzhu, LIU Wenwen, LI Guihua,GUO Zixuan, JIAO Ziwei   

  1. School of Biological Sciences and Technology, Yili Normal University, Yining 835000, China
  • Online:2024-10-18 Published:2024-10-14

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摘要： 为探究不同种植年限对新疆伊犁薰衣草根系分泌物的影响，采用液相色谱-质谱联用仪(LC-MS)对种植1年、3年和5年薰衣草(L1、 L3和L5)的根际土壤，以及未种植薰衣草（L0）的土壤进行非靶向代谢组分析。结果表明，根系分泌物包括有机酸类、脂类、核酸类、碳水化合物类、维生素类和辅助因子类、激素类、多肽类、抗生素类和类固醇类。相较L0，L1、L3、L5分别筛选出26、17与21种显著差异分泌物（P＜0.001）；L1、 L3和L5分别富集到13、10条和9条显著差异代谢通路（P＜0.05）。相较L1，L3和L5分别筛选到27种（P＜0.05）和9种显著差异分泌物（P＜0.001）；L3和L5分别富集到4条和9条显著差异代谢通路（P＜0.05）。与L3相比，L5筛选到13种显著差异分泌物（P＜0.05），富集到6条显著代谢通路（P＜0.05）。总体来说，随着种植年限的延长，甾醇类物质先下降后上升，维生素类、多肽类和核苷酸类逐渐下降，薰衣草根系分泌物发生显著变化。

关键词: 根系分泌物, 非靶向代谢组, 薰衣草, 连续种植, 根际

Abstract: To explore the impact of different cultivation periods on the exudates ofLavandula angustifoliain Yili, Xinjiang, non-targeted metabolomic analysis was conducted by using liquid chromatography-mass spectrometry (LC-MS) on the rhizosphere soil samples of 1-year-old, 3-year-old, and 5-year-old lavender plants(named L1, L3, and L5), as well as soil samples without lavender cultivation (L0). The results indicated that root exudates included nine main categories: organic acids, lipids, nucleic acids, carbohydrates, vitamins and cofactors, hormones, peptides, antibiotics, and steroids. Compared to L0, L1, L3, and L5 resulted in the identification of 26, 17, and 21 significantly different exudates, respectively (P＜0.001). Additionally, L1, L3, and L5 exhibited enrichment in 13, 10, and 9 significantly different metabolic pathways (P＜0.05), respectively. Compared to L1, L3 and L5 showed 27 (P＜0.05) and 9 significantly different exudates (P＜0.001), respectively, and enrichment in 4 and 9 significantly different metabolic pathways (P＜0.05), respectively. Furthermore, when compared to L3, L5 presented 13 significantly different exudates (P＜0.05) and enrichment in 6 significantly different metabolic pathways (P＜0.05). Overall, the exudates ofLavandula angustifoliaroot system underwent significant changes as the cultivation period extended, with sterols initially decreasing and then increasing, while vitamins, peptides, and nucleotides gradually decreased.

Key words: root exudate, untargeted metabolome, lavender, continuous planting, rhizosphere

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