生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (1): 63-.doi: 10.3969/j.issn.2095-1736.2020.01.063

• 研究报告 • 上一篇    下一篇

南方根结线虫侵染下罗汉果代谢组学研究

  

  1. 1. 广西师范大学 生命科学学院， 桂林 541006; 2. 广西师范大学珍稀濒危动植物生态与环境保护教育部重点实验室, 桂林 541006 
  • 出版日期:2020-02-18 发布日期:2020-03-09
  • 通讯作者: 李惠敏，硕士，副教授，从事分子生物学教学与研究工作，E-mail：huiminli@mailbox.gxnu.edu.cn
  • 作者简介:李璐璐，硕士研究生，研究方向为植物分子生物学，E-mail：1743957861@qq.com
  • 基金资助:
    广西高校中青年教师基础能力提升项目（2018KY0084）；广西自然科学基金青年基金（2016GXNSFBA380061）；广西师范大学青年骨干教师成长支撑计划项目(201317C)

Metabonomics analysis of Siraitia grosvenorii under infection of Meloidogyne incognita

  1. 1. College of Life Science，Guangxi Normal University， Guilin 541006; 2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin 541006, China
  • Online:2020-02-18 Published:2020-03-09

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摘要： 罗汉果为广西特有经济作物，但其主栽品种易受根结线虫的侵害，而对于罗汉果植株根系受根结线虫侵染早期其代谢变化情况知之甚少。基于LC-MS 技术的代谢组学方法，对南方根结线虫侵染下的罗汉果根系进行了代谢轮廓变化非靶向分析。主成分分析（PCA）和正交偏最小二乘判别分析(OPLS-DA)结果表明，接种南方根结线虫7 d后，是否受到根结线虫侵染的罗汉果之间其根系代谢物呈现明显分离。单变量分析和t检验分析结果显示，有16种代谢物含量发生显著性差异变化，这些代谢物主要涉及糖代谢、脂类代谢和氨基酸代谢。结果可为进一步揭示南方根结线虫与罗汉果根系互作的分子机制提供参考和研究思路。

关键词: 罗汉果, 南方根结线虫, 代谢组, 液相色谱-质谱联用技术

Abstract: Siraitia grosvenii is a special economic crop in Guangxi Autonomous Region and its main varieties are susceptible to root-knot nematodes， but the change of metabolites of Siraitia grosvenorii infected by M. incognita in the early stage was poorly understood. In this study, non-target analysis based on liquid chromatography-mass spectrometry was used to analyze the metabolic profile of the roots of Siraitia grosvenii seedlings infected by M. incognita. Principal component analysis and orthogonal partial least-squares discrimination analysis were also carried out for pattern recognition. The result showed that a clear separation was achieved between the control and treated with M. incognita at 7 days post-inoculate. Statistical significance was assessed with a t-test and a 2-fold change and 16 metabolites in root extracts of Siraitia grosvenii changed significantly in the level P<0.05. These metabolites were mainly related to glucose metabolism, lipid and amino acid metabolism. The result here can provide insight into interaction between Siraitia grosvenii and M.incognita in early phase.

Key words: Siraitia grosvenorii, Meloidogyne incognita, metabonomics, LC-MS

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