生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (6): 47-.doi: 10.3969/j.issn.2095-1736.2024.06.047

• 研究报告 • 上一篇    下一篇

云南省地生兰根内微生物多样性分析

吴峰婧琳1， 陈健鑫1， 杨娅琳1， 姬靖捷1， 张东华1， 马焕成2， 伍建榕1，2   

  1. 1. 西南林业大学 林学院 云南省高校森林灾害预警控制重点实验室， 昆明 650224；
    2. 西南林业大学 林学院 西南地区生物多样性保育国家林业和草原局重点实验室， 昆明 650224
  • 出版日期:2024-12-18 发布日期:2024-12-16
  • 通讯作者: 伍建榕，教授，主要从事森林病理和资源微生物利用研究，E-mail:1176279044@qq.com
  • 基金资助:
    国家自然科学基金项目（31860208）；国家重点研发计划项目（2019YFD100200X）；西南林业大学木棉纤维人工林产业化培育省级创新团队项目（2018HC014）

Analysis of microbial diversity in the roots of terrestrial orchids in Yunnan Province

WU Fengjinglin1, CHEN Jianxin1, YANG Yalin1, JI Jingjie1, ZHANG Donghua1,MA Huancheng2, WU Jianrong1,2   

  1. 1. Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province, College of Forestry,
    Southwest Forestry University, Kunming 650224, China; 2. Key Laboratory of National Forestry and Grassland
    Administration on Biodiversity Conservation in Southwest China，College of Forestry, Southwest Forestry University,
    Kunming 650224, China
  • Online:2024-12-18 Published:2024-12-16

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摘要： 为探究不同生长条件下地生兰根内微生物的分布情况，通过宏基因组测序技术分析不同生长条件下根内微生物群落差异，并对其进行潜在功能预测。结果表明，野生状态下地生兰根内微生物的丰富度和多样性均高于组培状态下，且野生地生兰根内微生物主要核心菌群为变形菌门（Proteobacteria）、放线菌门（Actinomycetota）和子囊菌门（Ascomycota），组培状态下的核心菌群为变形菌门、担子菌门（Basidiomycota）和病毒类（Viruses noname）。此外，野生状态下和组培状态下兰科菌根真菌（orchid mycorrhiza fungi， OMF）的主要优势类群也存在显著差异。丛枝菌根真菌（arbuscular mycorrhizal fungi， AMF）在组培状态下相对丰度显著高于野生状态下。通过对2种不同生长条件下根内微生物代谢功能进行预测，结果发现，野生状态下碳水化合物代谢（carbohydrate metabolism）、氨基酸代谢（amino acid metabolism）、能量代谢（energy metabolism）以及辅因子和维生素代谢（metabolism of cofactors and vitamins）等功能基因较为丰富。该研究结果为地生兰的保育、组培苗菌根化培育和病虫害防治提供了理论依据。

关键词: 地生兰, 根内微生物, 宏基因组, 野生兰花, 栽培兰花

Abstract: In order to investigate the distribution of microorganisms in the roots of terrestrial orchids under different growth conditions, the differences of microbial communities in the roots under different growth conditions were analyzed by metagenomic sequencing technology, and the potential functions of these microorganisms were predicted. The results showed that the abundance and diversity of microorganisms in the roots of wild orchids were higher than that of those ochids in the cultivated condition. The main groups of microorganisms in the roots of orchids in the wild condition were Proteobacteria, Actinobacteria, and Ascomycota, while those in the cultivated condition were Proteobacteria, Basidiomycota and Viruses noname. In addition, there were significant differences in the main dominant taxa of orchid mycorrhiza fungi （OMF）in the wild and in the cultivated condition. The relative abundance of arbuscular mycorrhizal fungi （AMF） was significantly higher in the cultivation than in the wild. By predicting the metabolic functions of microorganisms in the roots under two growth conditions, it was found that functional genes for carbohydrate metabolism, amino acid metabolism, energy metabolism and metabolism of cofactors and vitamins were more abundant in the wild condition. The results of this study provided a theoretical basis for the conservation, artificial mycorrhizalization seedling cultivation, and pest control of terrestrial orchids.

Key words: terrestrial orchids, root endospheres, macrogenome, wild orchids, cultivation orchids

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