生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (3): 51-.doi: 10.3969/j.issn.2095-1736.2025.03.051

• 研究报告 • 上一篇    下一篇

多花黄精调控多糖生物合成途径的bHLH转录因子家族鉴定与分析

王郅炜1,2， 张京晶1,2， 单婷玉1,2， 钟欣欣1,2， 樊金世博1,2， 程　卉2,3， 吴家文2,3,4   

  1. 1. 安徽中医药大学 研究生院， 合肥 230012; 2. 安徽中医药大学新安医学教育部重点实验室，
    合肥 230038; 3. 安徽省中医药科学院， 合肥 230012; 4. 安徽道地中药材品质提升协同创新中心，
    合肥 230012
  • 出版日期:2025-06-18 发布日期:2025-06-16
  • 通讯作者: 程卉，博士，副研究员，研究方向为中药及活性成分抗肿瘤，E-mail:chenghuikyzx@ahtcm.edu.cn；吴家文，博士，教授，研究方向为中草药分子生物学，E-mail:wujiawen@ahtcm.edu.cn；程卉和吴家文为共同通信作者
  • 作者简介:王郅炜，硕士，研究方向为中草药分子生物学，E-mail:wzwei720@163.com
  • 基金资助:
    安徽省高等学校科学研究项目（2022AH050474）； 安徽省自然科学基金项目（2308085MH305， 2008085MH268）； 国家重点研发项目（2017YFC1701600）

Identification and analysis of the bHLH transcription factor family involved in polysaccharide biosynthesis pathway in Polygonatum cyrtonema

WANG Zhiwei1,2, ZHANG Jingjing1,2, SHAN Tingyu1,2, ZHONG Xinxin1,2, FAN Jinshibo1,2,CHENG Hui2,3, WU Jiawen2,3,4   

  1. 1. Graduate School of Anhui University of Chinese Medicine, Hefei 230012, China; 2. Key Laboratory of Xin’an
    Medicine, Anhui University of Chinese Medicine, Hefei 230038, China; 3. Anhui Academy of Chinese Medicine,
    Hefei 230012, China; 4. Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,
    Hefei 230012, China
  • Online:2025-06-18 Published:2025-06-16

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摘要： 为探讨多花黄精bHLH转录因子（PcbHLH）在多糖生物合成途径中的调控作用，研究基于高通量测序对多花黄精的根茎、根、叶和花进行转录组分析，获得235个多花黄精bHLH转录因子，将它们的蛋白序列与拟南芥bHLH构建系统发育树，归类为25个亚家族。KEGG代谢通路分析表明，有15个PcbHLH涉及多糖生物合成途径，亚细胞定位分析表明，其中13个PcbHLH蛋白可能定位在细胞核。序列分析显示，α-螺旋和无规卷曲是它们二级结构的主要元件，空间结构上它们都具有2对不相连的\[由2个α-螺旋所组成的螺旋-环-螺旋（HLH）\]结构。基因表达分析发现15个PcbHLH中有9个在根茎中表达量最高，而蛋白质-蛋白质相互作用结果显示，PcbHLH与多糖生物合成途径中5个关键酶互作程度较高，表明PcbHLH很可能通过调控这些关键酶调节多糖生物合成。研究为进一步探究PcbHLH的结构和功能提供理论依据，为最终揭示bHLH如何调控多花黄精中的多糖生物合成途径奠定实验基础。

关键词: 多花黄精, 转录因子, bHLH, 转录组分析, 多糖生物合成

Abstract: In order to investigate the regulatory function of thePolygonatum cyrtonemabHLH transcription factor (PcbHLH) in the polysaccharide biosynthesis pathway, transcriptome sequencing was performed for the rhizome, root, leaf, and flower tissues ofP. cyrtonema. Based on transcriptome analysis, 235 PcbHLH transcription factors were identified. Phylogenetic trees were then constructed using their protein sequences and those ofArabidopsis thalianabHLH proteins, dividing the 235 PcbHLHs into 25 subfamilies. 15 PcbHLHs were related to the polysaccharide biosynthesis pathways using KEGG pathway analysis, and subcellular localization analysis indicated that 13 PcbHLHs of which were likely located in the nucleus. Sequence analysis demonstrated that α-helices and random coils were the primary components of their secondary structures. There were two helix-loop-helix (HLH) motifs in their spatial structures. Gene expression profile showed that 9 out of 15 PcbHLHs exhibited the highest expression levels in rhizomes. The study on protein-protein interaction demonstrated that PcbHLHs showed significant interactions with five key enzymes in the polysaccharide biosynthesis pathway, suggesting that PcbHLHs might regulate polysaccharide biosynthesis though these enzymes. This research will provide a theoretical foundation for further exploration for the structure and function of PcbHLHs, and establish an experimental basis for revealing the mechanisms of the polysaccharide biosynthesis pathway regulated by PcbHLH inP. cyrtonema.

Key words: Polygonatum cyrtonema, transcription factor, bHLH, transcriptome analysis, polysaccharide biosynthesis

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