生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (3): 47-.doi: 10.3969/j.issn.2095-1736.2025.03.047

• 研究报告 • 上一篇    下一篇

黄帚橐吾精油对黏虫幼虫的毒力及神经传导相关酶系的影响

李　晶， 刘锦霞， 李　娜， 付麟雲， 丁　品， 聂垚琰， 武建荣， 杨　成   

  1. 甘肃省科学院 生物研究所 甘肃省微生物资源开发利用重点实验室， 兰州 730000
  • 出版日期:2025-06-18 发布日期:2025-06-16
  • 通讯作者: 刘锦霞，研究员，研究方向为农业病虫害生物综合防治，E-mail:liujinx0168@163.com
  • 作者简介:李晶，硕士，副研究员，研究方向为生物农药研发，E-mail:eyre326@163.com
  • 基金资助:
    甘肃省科学院重点研发项目（2021JK-02）；甘肃省重点人才项目（2023）

Effects of Ligularia virgaurea essential oil on the toxicity and nerve transduction related enzymes of Mythimna separate larvae

LI Jing, LIU Jinxia, LI Na, FU Linyun, DING Pin, NIE Yaoyan, WU Jianrong, YANG Cheng #br#   

  1. Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province,Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China
  • Online:2025-06-18 Published:2025-06-16

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摘要： 研究黄帚橐吾精油对黏虫幼虫的毒性，并探讨黏虫幼虫神经传导相关酶系对黄帚橐吾精油胁迫的响应机制。通过点滴法测定黄帚橐吾精油对黏虫幼虫的毒性，结果显示：该精油具有强烈的生物活性，LC50为4.584 mg/mL。进一步采用酶联免疫吸附法测定不同处理时间下，精油对黏虫幼虫体内神经传导相关酶活性的影响。结果表明，LC50质量浓度的精油能显著抑制黏虫幼虫乙酰胆碱酯酶和Na+-K+-ATP酶活性。结果表明黄帚橐吾精油可能对黏虫幼虫具有神经毒性，具备开发成为新型生物农药的潜力。

关键词: font-size:9.00pt, line-height:150%, ">精油； 乙酰胆碱酯酶； Na+-K+-ATP酶； 生物农药； 神经毒性

Abstract: In this paper, the toxicity of Ligularia virgaurea essential oil to armyworm larvae was studied, and the response mechanism of nerve transduction related enzymes in armyworm larvae to the stress of L. virgaurea essential oil was investigated. The indoor toxicity was determined by leaf immersion method. As a result, L. virgaurea essential oil showed strong biological activity with LC50 of 4.584 mg/mL. Enzyme-linked immunosorbent assay (ELISA) was used to determine the effect of essential oil on nerve conduction-related enzyme activity in armyworm larvae at different time. The results revealed that essential oil at the mass concentration of LC50 could significantly inhibit the activities of acetylcholinesterase and Na+-K+-ATPase. These results assume that L. virgaurea essential oil may be neurotoxic to armyworm larvae, and hence be potential to be developed as a new bio-pesticide.

Key words: essential oil, AChE, Na+-K+-ATPase, bio-pesticide, neurotoxicity

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