生物学杂志

生物学杂志 ›› 2025, Vol. 42 ›› Issue (3): 36-.doi: 10.3969/j.issn.2095-1736.2025.03.036

• 研究报告 • 上一篇    下一篇

苦草和仿生水草附着细菌群落对不同氮形态的响应研究

盛梦婷1， 俞佳铭2， 程铁涵1， 曹玉成1,3， 周昕彦1,3   

  1. 1. 浙江农林大学 环境与资源学院、碳中和学院， 杭州 311300； 2. 浙江农林大学 集贤学院，
    杭州 311300； 3. 浙江农林大学 浙江-西班牙农业环境新污染物联合实验室， 杭州 311300
  • 出版日期:2025-06-18 发布日期:2025-06-16
  • 通讯作者: 周昕彦，副教授，研究方向为水污染治理与水生态修复，E-mail:zhouxy1993@zafu.edu.cn
  • 作者简介:盛梦婷，硕士研究生，研究方向为水污染治理与水生态修复，E-mail:2022603042034@stu.zafu.edu.cn
  • 基金资助:
    浙江省重大科技专项重点社会发展项目（2015C03007）； 浙江省重点研发计划项目（2019C03121）； 浙江农林大学科研发展基金项目（2020FR056）； 国家自然科学基金项目（22306168）

Responses of bacterial communities attached to Vallisneria natans and bionic aquatic plant to different aquatic nitrogen conditions

SHENG Mengting1, YU Jiaming2, CHENG Tiehan1, CAO Yucheng1,3, ZHOU Xinyan1,3   

  1. 1. College of Environment and Resources, College of Carbon Neutrality, Zhejiang A & F University,
    Hangzhou 311300, China; 2. Jixian Honors College, Zhejiang A & F University, Hangzhou 311300, China;
    3. Sino-Spain Joint Laboratory for Agricultural Environment Emerging Contaminants of Zhejiang Province,
    Zhejiang A & F University, Hangzhou 311300, China
  • Online:2025-06-18 Published:2025-06-16

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摘要： 利用16S rRNA基因高通量测序手段研究苦草和仿生水草附着细菌群落的结构和氮循环功能对水中3种氮形态（2 mg/L NH+4-N、1 mg/L NH+4-N+1 mg/L NO－3-N、2 mg/L NO－3-N）的响应规律。结果表明，单独NH+4-N处理下苦草附着生物膜的生物量最高。单独NO－3-N处理下沉水植物附着细菌群落α多样性最高，NH+4-N与NO－3-N共存处理对细菌群落β多样性的影响较大。沉水植物附着细菌群落中的优势菌属为假单胞菌、气单胞菌和不动杆菌，它们对氮形态的响应规律存在差异。单独NH+4-N处理下细菌共现网络的模块化程度最高，两种氮共存时细菌网络中物种间的竞争更强。PICURSt2功能预测表明，NO－3-N处理会降低硝化功能基因在苦草附着生物膜中的相对丰度。研究揭示了不同氮形态对沉水植物附着细菌群落结构及功能的影响规律，可为今后开发适应不同水质条件的沉水植物净化技术提供理论支撑。

关键词: 沉水植物, 附着细菌, 多样性, 群落组成, 氮循环功能基因

Abstract: High-throughput 16S rRNA gene sequencing was used in this study to dissect the bacterial community structure and nitrogen cycling functions in Vallisneria natans and bionic aquatic plant when subjected to three nitrogen forms in water: 2 mg/L NH+4-N, a combination of 1 mg/L NH+4-N and 1 mg/L NO－3-N, and 2 mg/L NO－3-N. The results indicated that V. natans supported the greatest biofilm biomass under NH+4-N exposure. Sole NO－3-N treatment resulted in the highest α diversity within the bacterial community attached to submerged plants, while the co-occurrence of NH+4-N and NO－3-N notably influenced on the community’s β diversity. Pseudomonas, Aeromonas, and Acinetobacter were identified as the dominant bacterial genera, exhibiting distinct responses to the different nitrogen conditions. Furthermore, the modularity of bacterial co-occurrence networks was most pronounced under NH+4-N treatment alone, whereas increased interspecies competition was observed with the combined nitrogen forms. PICRUSt2 function prediction indicated a reduction in the relative abundance of nitrification genes in the V. natans biofilm under NO－3-N treatment. These findings shed light on the impact of various nitrogen forms on the structures and functions of bacterial community on submerged plants, which is instrumental for the advancement of water purification techniques utilizing submerged vegetation across varying water quality scenarios.

Key words: submerged aquatic plants, attached bacteria, diversity, community composition, nitrogen cycle functional genes

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