生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (1): 46-.doi: 10.3969/j.issn.2095-1736.2023.01.046

• 研究报告 • 上一篇    下一篇

安徽地区主栽粳（糯）稻品种遗传多样性分析及DNA指纹图谱构建

周　燃1， 甘　泉2， 林翠香2， 宋丰顺2， 吴德祥1， 倪大虎2   

  1. 1. 安徽农业大学 农学院， 合肥 230036; 2. 安徽省农业科学院水稻研究所， 合肥 230031
  • 出版日期:2023-02-18 发布日期:2023-02-21
  • 通讯作者: 倪大虎，博士，研究员，研究方向为水稻分子遗传与新品种选育，E-mail: dahuni1974@163.com
  • 作者简介:周燃，硕士研究生，研究方向为水稻分子育种，E-mail: 2605795449@qq.com
  • 基金资助:
    安徽省重点研究与开发计划项目（202104f06020016）

Genetic diversity analysis and DNA fingerprint profile construction of major Japonica (Glutinous) rice varieties in Anhui Province

ZHOU Ran1, GAN Quan2, LIN Cuixiang2, SONG Fengshun2, WU Dexiang1, NI Dahu2   

  1. 1. College of Agronomy, Anhui Agricultural University, Hefei 230036, China;
    2. Rice Research Institute, Anhui Academy of Agricultural Science, Hefei 230031, China
  • Online:2023-02-18 Published:2023-02-21

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摘要： 以安徽省近年来主栽的21份粳（糯）稻品种为材料，采用国家标准NY/T 1433—2014中推荐的48对SSR引物进行多态性分析，研究品种间的遗传多样性。48对引物中21个SSR标记在21份材料中存在多态性，共检测到59个等位基因，平均每个标记检测到2.81个。遗传多样性分析显示：Shannon’s多样性指数变幅为0.199~1.163，平均值为0.657；Nei’s指数变幅为0.095~0.620，平均值为0.390。多态信息含量（PIC）处于0.091~0.549，平均值为0.331。21个品种间存在一定遗传差异，但等位基因观测数、Shannon’s指数、Nei’s指数均较低，表明21个品种间遗传背景较为狭窄。UPGMA聚类分析表明：21个粳（糯）稻品种的遗传相似系数为0.426~0.954，遗传相似系数在0.62处可将21个品种分为5个类群。根据标记的PIC值和MI值进一步简化标记的使用量，利用7个核心SSR标记构建了21份粳（糯）稻材料的标准指纹图谱，为品种真实性判别、纯度鉴定以及核心种质提纯奠定基础。

关键词: 粳（糯）稻, 遗传多样性, SSR标记, DNA指纹图谱

Abstract: Twenty-one japonica (glutinous) rice varieties, which have been mainly planted in Anhui Province in recent years, were used as materials for polymorphism analysis according to the national standard (NY/T 1433—2014). Among the 48 SSR markers, 21 primers exhibited polymorphic between 21 materials, and a total of 59 alleles were detected, with an average of 2.81 per marker. The genetic diversity analysis showed that the variation of Shannon’s diversity index ranged from 0.199 to 1.163, with a mean value of 0.657; the variation of Nei’s index ranged from 0.095 to 0.620, with a mean value of 0.390. The polymorphic information content (PIC) ranged from 0.091 to 0.549,with a mean value of 0.331. There were some genetic differences among the 21 varieties.But, the observed number of alleles, Shannon’s index and Nei’s index, were low, indicating that the genetic background of the 21 varieties was narrow. The genetic similarity coefficients ranged from 0.426 to 0.954. The 21 varieties could be classified at 0.62 into five taxa. Based on the PIC and MI values of the markers, the polymorphic markers were further simplified and the standard fingerprint profiles of 21 japonica (glutinous) rice materials with 7 core SSR markers were constructed, which were prepared the ground for variety authenticity test, purity identification and core germplasm purification.

Key words: Japonica (glutinous) rice, genetic diversity, SSR marker, DNA fingerprinting

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