生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (2): 74-.doi: 10.3969/j.issn.2095-1736.2024.02.074

• 研究报告 • 上一篇    下一篇

基于mtDNA COI基因序列的山香圆平背粉虱遗传多样性分析

孟泽洪1， 王威锐1, 2， 罗林丽3， 李　帅1， 蒲运丹1， 周玉锋1   

  1. 1. 贵州省农业科学院茶叶研究所， 贵阳 550006； 2. 贵州大学 茶学院， 贵阳 550025；
    3. 贵州省农业科学院生物技术研究所， 贵阳 550006
  • 出版日期:2024-04-18 发布日期:2024-04-17
  • 通讯作者: 周玉锋，博士，研究员，研究方向为茶树病虫防控，E-mail:gzzhouyf@163.com
  • 作者简介:孟泽洪，博士，副研究员，研究方向为茶树害虫及生物学，E-mail:mengzehonguizhou@163.com
  • 基金资助:
    国家自然科学基金项目（31860227）； 黔农科院国基后补助〔2021〕27号； 贵州省农科院专项（黔农科院青年基金2018\[07\]号）

Genetic diversity analysis of tea insect pest Crenidorsum turpiniae based on mtDNA COI gene partial sequence

MENG Zehong1, WANG Weirui1, 2, LUO Linli3, LI Shuai1, PU Yundan1, ZHOU Yufeng1   

  1. 1. Institute of Tea, Guizhou Academy of Agricultural Science, Guiyang 550006, China; 2. Tea College,
    Guizhou University, Guiyang 550025, China; 3. Institute of Biotechnology, Guizhou Academy of Agricultural Sciences,
    Guiyang 550006, China
  • Online:2024-04-18 Published:2024-04-17

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摘要： 为探究茶树害虫山香圆平背粉虱（Crenidorsum turpiniae）的遗传多样性，掌握山香圆平背粉虱的遗传分化现状及精准防控提供理论依据，通过基因组DNA提取、PCR扩增与测序，测定贵州省11个不同地理区域的山香圆平背粉虱种群mtDNA COI基因序列片段，开展基因序列特征、单倍型及遗传多样性、遗传分化与基因交流、分子变异和单倍型系统发育分析。结果表明，山香圆平背粉虱mtDNA COI基因序列片段扩增长度均为658 bp，序列具有明显的A+T偏倚性，共定义10个单倍型。贵州茶园山香圆平背粉虱总群体遗传多样性较高，表现出高单倍型多样性（Hd=0.800）和高核苷酸多样性（Pi=0.0670）。11个地理种群的山香圆平背粉虱总群体遗传分化程度高（Fst =0.9203），基因交流水平低（Nm=0.04）。AMOVA分析显示，山香圆平背粉虱的遗传变异主要来自组间（FCT=0.9129）；中性检验与错配分布分析综合表明，山香圆平背粉虱近期未发生过明显扩张现象；单倍型系统发育分析及中介网络图聚类结果均表明，山香圆平背粉虱聚为3分支，分化枝可能已达物种水平。

关键词: 茶树新害虫, 山香圆平背粉虱, 线粒体COI基因, 遗传多样性, 遗传分化

Abstract: To investigate the genetic diversity of the tea pest C. turpiniae and ascertain the degree of genetic differentiation and provide a theoretical basis for control, genomic DNA of C. turpiniae populations from 11 different geographical regions in Guizhou Province was extracted. It was amplified through PCR and sequenced in order to determine the mtDNA COI gene fragments. Subsequently, analysis of the gene sequence characteristics, haplotypes and genetic diversity, genetic differentiation and gene flow, molecular variation and haplotype phylogenetic analysis was conducted. The results showed that the mtDNA COI gene sequence fragment amplified from C. turpiniae was 658 bp in length, and the sequence showed obvious A+T bias. A total of 10 haplotypes were defined. The total population of C. turpiniae in Guizhou tea gardens had a high level of genetic diversity, high haplotype diversity (Hd=0.800) and high nucleotide diversity (Pi=0.0670). The total population of the eleven geographical populations had a high degree of genetic differentiation (Fst=0.9203) and low level of gene communication (Nm=0.04). The results of the AMOVA analysis showed the majority of the genetic variation in C. turpiniae population due to differences between groups (FCT=0.9129). The comprehensive analysis of neutral test and mismatch distribution showed that the population had not expanded significantly recently. The results of both haplotype phylogenetic analysis and haplotype network clustering showed that C. turpiniae population was clustered into three clades.

Key words: tea newly recorded pest, Crenidorsum turpiniae, mtDNA COI gene, genetic diversity, genetic differentiation

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