生物学杂志

生物学杂志 ›› 2020, Vol. 37 ›› Issue (5): 44-.doi: 10.3969/j.issn.2095-1736.2020.05.044

• 研究报告 • 上一篇    下一篇

线叶龙胆（Gentiana lawrencei var. farreri）ITS序列杂合性与遗传分化分析

  

  1. 洛阳师范学院 生命科学学院， 洛阳 471934
  • 出版日期:2020-10-18 发布日期:2020-10-14
  • 通讯作者: 孙姗姗，博士，讲师，主要从事植物适应性进化研究，E-mail: sss823129@126.com
  • 作者简介:付鹏程，博士，副教授，主要从事植物进化研究，E-mail: fupengc@sina.com
  • 基金资助:
    国家自然科学基金（31600296）

Analysis of ITS heterozygosity and genetic divergence inGentiana lawrencei var. farreri

  1. School of Life Science, Luoyang Normal University, Luoyang 471934, China
  • Online:2020-10-18 Published:2020-10-14

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摘要： 线叶龙胆（Gentiana lawrencei var. farreri）是龙胆属的常见种之一，分布于青藏高原地区，具有药用与观赏价值。ITS是重要的分子标记之一，可用于植物群体遗传与进化研究；然而由于其杂合性较高，为后续分析带来一定困难。以线叶龙胆7个居群共39个个体为对象，通过基因克隆与测序，共得到81条序列，鉴定出76个单倍型。结果表明ITS序列在线叶龙胆中存在很高的杂合性，居群遗传多样性高；且遗传变异的87.61%来自居群内，仅有12.39%来自于居群间。在鉴定的单倍型中，97.36%为单个个体所特有，个体间很少共享同一种单倍型；进一步的系统发育分析表明这些单倍型无法聚成高支持率的支系。研究揭示了线叶龙胆ITS序列存在高度杂合性，可为龙胆属的进化研究提供参考。

关键词: 线叶龙胆, ITS, 杂合性, 遗传分化

Abstract: Gentiana lawrencei var. farreri, which has medical and gardening value, is a common species in Gentiana and distributes on the Qinghai-Tibetan Plateau. Nuclear ribosomal internal transcribed spacer (ITS) is a common molecular marker used in phylogenetics and population genetics， but has difficulty in sequencing and data analysis due to heterozygosity. A total of 39 individuals from 7 populations of G. lawrencei var. farreri were sequenced after cloning in this study. The obtained 81 sequences identify 76 haplotypes in total. For the genetic variations, 87.61% occured within population and only 12.39% occured among populations. Haplotypes, which were rarely shared among individuals （2.64%）, did not show a clear phylogenetic relationship. In conclusion, this study showed high ITS heterozygosity in G. lawrencei var. farreri and would be helpful in researches about Gentiana evolution.

Key words: Gentiana lawrencei var. farreri, ITS, heterozygosity, genetic divergence

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