生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (2): 80-.doi: 10.3969/j.issn.2095-1736.2023.02.080

• 研究报告 • 上一篇    下一篇

红螯螯虾雌雄间性个体性腺转录组特征分析

李　飞， 郑建波， 程　顺， 刘士力， 刘一诺， 贾永义， 迟美丽， 顾志敏   

  1. 浙江省淡水水产研究所 农业农村部淡水渔业健康养殖重点实验室 
    浙江省淡水水产遗传育种重点实验室， 湖州 313001
  • 出版日期:2023-04-18 发布日期:2023-04-18
  • 通讯作者: 顾志敏，研究员，研究方向为水产养殖与遗传育种，E-mail:guzhimin2006@163.com
  • 作者简介:李飞，硕士，副研究员，研究方向为水产动物遗传育种与繁殖，E-mail:lifeibest1022@163.com
  • 基金资助:
    国家重点研发计划项目（2018YFD0901305）； 国家淡水水产种质资源库长江流域种质资源分库建设项目

Characteristics of the gonadal transcriptiome of intersex red claw crayfish Cherax quadricarinatus

LI Fei, ZHENG Jianbo, CHENG Shun, LIU Shili, LIU Yinuo, JIA Yongyi,CHI Meili, GU Zhimin   

  1. Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and
    Breeding of Zhejiang Province, Ministry of Agriculture and Rural Affairs, Zhejiang Institute of Freshwater Fisheries,
    Huzhou 313001, China
  • Online:2023-04-18 Published:2023-04-18

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摘要： 为研究红螯螯虾雌雄间性个体与正常个体之间的差异，对红螯螯虾雌雄间性个体和正常个体的精巢和卵巢运用Illumina Novaseq 6000进行转录组测序，同时借助生物信息学开展功能基因预测和基因表达对比分析。通过测序和组装，共获得83.79 Gb质控碱基，各样品质控碱基均达到6.29 Gb以上，Q30碱基百分比在94.25%以上，组装得到的Unigene个数为53077，转录本个数为73588。基因/转录本与6大数据库比对上的比例为18.17%～36.07%。基因表达分析结果表明：样本间共表达的Unigene数为3543个，不同个体卵巢的表达量相关系数最大，不同个体精巢的表达量相关系数次之，卵巢和精巢之间表达量相关系数最小；正常个体的卵巢和精巢与不同雌性间性个体的精巢和卵巢的基因表达量存在差异，累计差异表达的基因有310个。研究认为，红螯螯虾雌雄间性个体不仅在外部形态上与正常个体存在差异，在性腺的转录水平上也存在着差异，且不同类型的雌雄间性个体与正常个体之间的差异程度不同。

关键词: 红螯螯虾, 雌雄间性, 转录组, 特征, 差异分析

Abstract: In order to study the difference between the intersex and normal individuals, their testis and ovary were sequenced with Illumina Novaseq 6000 in this study, and were analyzed and compared in biological information and gene expression level. With sequencing, 83.79 Gb clean reads were obtained in total, and 6.29 Gb on average, the Q30 bases percentage was more than 94.25%. After assembling, 53077 Unigenes and 73588 transcripts were obtained, then they were mapped with the six international data, the mapping ratio was 18.17%-36.07%. The gene expression analyzing indicated that there were 3543 Unigenes co-expressed, the correlation of expression between ovaries was the greatest, the correlation for testis was followed, the correlation between ovary and testis was the last one. There were differences in the amount of expression gene between normal and intersex individuals, with 310 genes in total. In conclusion, besides differences in morphology between intersex and normal individuals, there were differences in transcription level for gonads, and the level was different for different kinds of intersex individuals.

Key words: red claw crayfish, intersex, transcription, characteristics, different analysis

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