生物学杂志 ›› 2020, Vol. 37 ›› Issue (2): 29-.doi: 10.3969/j.issn.2095-1736.2020.02.029

• 研究报告 • 上一篇    下一篇

鸟类SLC2A4基因及其编码蛋白GLUT4分子进化分析

  

  1. 安徽医科大学 生命科学学院, 合肥 230032
  • 出版日期:2020-04-18 发布日期:2020-04-17
  • 通讯作者: 刘晓颖,副教授,硕士生导师,主要研究方向为肿瘤细胞生物学,E-mail:liuxiaoying@ahmu.eud.cn
  • 作者简介:刘刚,博士,硕士生导师,主要研究方向为进化与分子遗传学,E-mail:liugang8966@163.com
  • 基金资助:
    国家自然科学基金项目(31702030,81201368)

Molecular evolution of SLC2A4 and its coding protein GLUT4 in aves

  1. School of Life Sciences, Anhui Medical University, Hefei 230032, China
  • Online:2020-04-18 Published:2020-04-17

摘要: 鸟类需要高效的血糖调节机制来维持飞翔时所需要的大量能量,SLC2A4基因及其表达产物GLUT4是细胞吸收葡萄糖的重要转运蛋白,该基因对鸟类飞翔中的能量利用具有重要意义。从GenBank中下载了17种鸟类SLC2A4基因序列进行分析,发现该基因的长度、碱基组成均具有较高的变异性;生物信息学分析显示GLUT4的信号肽剪切位点在氨基酸序列的第11位与57位之间,蛋白质分子具有亲水性,不存在跨膜结构且具有较多的O-糖基化位点;对基因的选择压力分析表明,突胸总目鸟类具有明显的正选择效应,而平胸总目鸟类具有纯化选择作用。采用最大似然法和贝叶斯法进行分析,系统发育分析结果显示系统发育树分为两枝:平胸总目分为第一枝,包括Struthioniformes、Apterygiformes和Tinamiformes 3个目;突胸总目构成第二枝,即Galliformes、Anseriformes、Columbiformes和Passeriformes,结果与形态学分类类似且获得了较高的支持率。研究结果将为鸟类的GLUT4进化研究提供有用的信息。

关键词: SLC2A4基因, GLUT4, 序列特征, 进化速率, 系统发育

Abstract: Birds need high-efficiency mechanism of blood glucose regulation to get energy for flying. SLC2A4 and its coding protein Glucose transporter type 4 (GLUT4) play a major role for glucose transport in skeletal muscle cells when birds fly. In this study, all of the 17 avian SLC2A4 sequences were got from GenBank, results showed that the sequences had high variation in the length and base composition. It also showed all the GLUT4 were hydrophilia and had many O-glycosylation sites, but had no transmembrane structure, and signal peptide sites were located in 11-57 site of GLUT4. Selection pressure analysis showed that it was positive selection in Carinatae, but negative selection in Ratitae. Using maximum likelihood (ML) and Bayesian inference (BI) methods based on the GLUT4, both of the trees were highly similar topologies and high bootstrap proportion values. The phylogenetic analysis showed that Struthioniformes, Apterygiformes and Tinamiformes formed together, then grouped with Galliformes, Anseriformes, Columbiformes and Passeriformes. Our results may provide useful information about the evolution of SLC2A4 in birds.

Key words: SLC2A4, GLUT4, sequence characterization, evolutionary rate, phylogeny

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