生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (6): 66-.doi: 10.3969/j.issn.2095-1736.2022.06.066

• 研究报告 • 上一篇    下一篇

利用流式细胞仪对猕猴桃种质染色体倍性的鉴定

  

  1. 1. 吉首大学 生物资源与环境科学学院， 吉首 416000；
    2. 广西壮族自治区中国科学院广西植物研究所， 桂林 541006
  • 出版日期:2022-12-18 发布日期:2022-12-12
  • 通讯作者: 王发明，博士，副研究员，主要从事果树分子遗传育种，E-mail：wfm_rz@163.com；刘世彪，博士，教授，主要从事植物资源研究，E-mail：liushibiao_1@163.com
  • 作者简介:罗庆，硕士研究生，主要从事猕猴桃物种研究，E-mail：1169623764@qq.com
  • 基金资助:
    国家自然科学基金地区科学基金项目(32060666); 广西重点研发计划项目（桂科AB16380091）; 广西植物研究所基本业务费项目(桂植业19002); 国家现代农业产业技术体系广西创新团队建设专项(nycytxcxtd-13-3)； 湖南省教育厅科学研究项目（20K102， 19K076）

Identification of chromosome ploidy in kiwifruit germplasm by flow cytometer

  1. 1. College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China; 2. Guangxi Zhuang
    Autonomous Region and Chinese Academy of Sciences, Guangxi Institute of Botany, Guilin 541006, China
  • Online:2022-12-18 Published:2022-12-12

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摘要： 为鉴定不同猕猴桃种质的染色体倍性，利用流式细胞仪对收集于广西桂林的50份猕猴桃种质材料进行倍性鉴定，其中有16份种质倍性为首次鉴定，并分析倍性与果实主要性状的相关性。结果表明：50份材料中共鉴定出二倍体猕猴桃19份，四倍体13份，六倍体18份，其中中华猕猴桃二倍体和四倍体较多，果肉颜色多红色或黄色，果面多被茸毛或光滑无毛；美味猕猴桃六倍体较多，果肉一般为绿色，多被长茸毛；美味猕猴桃的果实普遍大于中华猕猴桃。野生物种多为二倍体，绿色果肉，短茸毛至无毛。研究丰富了猕猴桃种的倍性数据库，为猕猴桃的育种和资源开发提供参考资料。

关键词: 猕猴桃, 染色体, 倍性鉴定, 果实特征, 流式细胞仪

Abstract: Ploidy identification is one of the preconditions for kiwifruit breeding. In this study, 50 kiwifruit germplasms were collected from Guilin, Guangxi Province, and their ploidy was identified using flow cytometry. Among them, the ploidy of 16 germplasms was identified for the first time, and the relationship between ploidy and fruit main features was analyzed. The results showed that 19 diploid, 13 tetraploid and 18 hexaploid were identified from the 50 species and varieties, the majority of Actinidia Chinensis were diploids and tetraploids, with red or yellow-fleshed and smooth or short trichomes on the fruit surface, while that of A.deliciosa was hexaploidy, with generally green-fleshed and long velutinous. The fruit size of A.deliciosa was larger than that of A. chinensis totally. Wild species were mostly diploids, accompanied with green-fleshed and smooth or less trichomes. This study enriched the ploidy database, and provided references for breeding and production of kiwifruit.

Key words: Actinidia, chromosome, ploidy identification, fruit feature, flow cytometry

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