生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (1): 33-.doi: 10.3969/j.issn.2095-1736.2021.01.033

• 研究报告 • 上一篇    下一篇

胸腺肽诱导Hela细胞死亡的研究

  

  1. 渭南师范学院 环境与生命科学学院, 渭南 714009
  • 出版日期:2021-02-18 发布日期:2021-02-22
  • 作者简介:李亚琳，博士，教授，主要从事肿瘤生物学研究，E-mail: lyal1222@126.com
  • 基金资助:
    陕西省教育厅实验室重点项目（16JS032）；渭南师范学院校级项目（15YKS004）

A study on Hela cells death induced by thymosin

  1. College of Environmental and Life Sciences, Weinan Normal University, Weinan 714009, China
  • Online:2021-02-18 Published:2021-02-22

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摘要： 为了研究胸腺肽Tα1对肿瘤细胞的生长抑制效应，试验检测了Tα1在体外对宫颈癌Hela细胞生长增殖的影响。不同浓度Tα1处理Hela细胞24 h或48 h，用显微镜观察Hela 细胞生长的形态学变化， MTT法检测细胞死亡率。采用实时定量PCR以及Western Blot检测自噬相关因子lc3基因及其蛋白的表达，进一步了解细胞死亡的行为。结果表明，用不同浓度的Tα1处理Hela细胞，在10~30 μg/mL 浓度范围内，随着Tα1含量的增高，Hela细胞的死亡率明显增加，同时LC3的表达量也显著升高，且与药物剂量呈正相关。这些结果说明Tα1在一定剂量范围内，能够在体外通过自噬的方式诱导Hela细胞死亡。

关键词: Hela细胞, Tα1, 肿瘤, 自噬

Abstract: In order to investigate the growth inhibition effect of thymosin alpha 1(Tα1) on tumor cells, this study detected the effect of Tα1 on the growth and proliferation of Hela cells of cervical cancer in vitro. The morphology of Hela cells was observed by microscope, the survival and growth of the cells were assayed by MTT. PCR and Westorn Blot were used to detect the expression of lc3 after the Hela were treated with Tα1 in different concentrations. The results showed that Tα1 induced the death of Hela and expression of lc3, and the expression quantity of lc3 were positively related with Tα1 concentration within a certain range. From the finding， we suggested that Tα1 induce the Hela cells death in vitro by autophagy probably.

Key words: Hela cell, thymosin alpha 1, tumor, autophagy

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