生物学杂志

生物学杂志 ›› 2021, Vol. 38 ›› Issue (1): 36-.doi: 10.3969/j.issn.2095-1736.2020.06.036

• 研究报告 • 上一篇    下一篇

植物乳杆菌UD01对具核梭杆菌生物膜形成的抑制

钟焱婷, 杨虹   

  1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室, 上海 200240
  • 出版日期:2021-02-18 发布日期:2021-02-22
  • 通讯作者: 杨虹，教授，博士，研究方向为益生乳酸细菌，E-mail：hongyang@sjtu.edu.cn
  • 作者简介:钟焱婷，硕士研究生，研究方向为益生乳酸细菌，E-mail：1529769935zyt@sjtu.edu.cn
  • 基金资助:
    国家自然科学基金项目（21477077）

Inhibition of Lactobacillus plantarum UD01 on biofilmformation of Fusobacterium nucleatum

ZHONG Yanting, YANG Hong   

  1. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2021-02-18 Published:2021-02-22

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摘要： 为了筛选在口腔中性pH条件下仍能抑制具核梭杆菌生物膜形成的益生菌，用源于不同益生菌的 pH 6.6 无细胞发酵上清液（Cell-free supernatant，CFS）处理具核梭杆菌，通过结晶紫半定量法测试其抑制具核梭杆菌生物膜形成的能力，从而筛选出一株抑制效果突出的植物乳杆菌UD01。微量倍比稀释法测定UD01CFS（pH 6.6）对具核梭杆菌生物膜形成的最低抑制浓度（The minimum biofilm inhibition concentration，MBIC）为250 μL/mL。扫描电镜和激光共聚焦显微镜结果显示：UD01 CFS（pH 6.6）处理下具核梭杆菌生物膜结构疏松、厚度变薄。生长曲线、外膜黏附相关基因表达以及胞外聚合物（Extracellular polymeric substances, EPSs）含量变化表明，UD01 CFS（pH 6.6）并不能抑制具核梭杆菌生长，其可能的作用机制是通过下调黏附相关外膜蛋白基因的表达、减少胞外蛋白和多糖的分泌，从而降低具核梭杆菌黏附能力，最终达到抑制生物膜形成的目的。

关键词: 口臭, 具核梭杆菌, 益生菌, 生物膜, 黏附能力

Abstract: In order to screen the probiotic which can inhibit the formation of Fusobacterium nucleatum biofilm under oral neutral pH, F. nucleatum was treated with pH 6.6 cell-free supernatant (CFS) from different probiotics, and its biofilm was determined by crystal violet semi-quantitative method.Lactobacillus plantarum UD01 was selected, due to its CFS(pH 6.6) could reduce the F. nucleatum biofilm.The minimum biofilm inhibition concentration (MBIC) was determined by the micro-dilution method, and the MBIC was 250 μL/mL. The results of SEM and CLSM showed that F.nucleatum biofilm decreased and became thinner, besides, its structure was looser after treated with UD01 CFS(pH 6.6). Growth curve, qPCR of adhesion-associated outer membrane protein genes, and extracellular polymeric substances (EPSs) experiments indicated that UD01 CFS (pH 6.6) could not inhibit the growth of F. nucleatum, and its possible action mechanism against F. nucleatum biofilm was to down-regulating the expression of adhesion-associated outer membrane protein genes, reduced the secretion of extracellular protein and polysaccharide, thereby reduced the adhesion ability of F. nucleatum, and ultimately inhibited F.nucleatum biofilm.

Key words: halitosis, Fusobacterium nucleatum, probiotics, biofilm, adhesion ability

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