关键词: 腐乳, 乳酸菌, 益生菌, 斑马鱼, 促生作用

Abstract: Probiotics play an important role in animal health, but the effects on the feed intake and moving capacity of animal larvae are not clear. Exploring the effects of probiotics on the growth and development of animals is of great significance for the regulation of larval nutrition. Traditional fermented foods are one of the sources of probiotics. In this study, a lactic acid bacteria (FR27) resistant to bile salts was isolated from fermented bean curd by using lactic acid bacteria culture medium (MRS). The strain was sequenced and the results showed this strain was affiliated to Lactobacillus plantarum. To explore the effect of FR27 on the growth and development of zebrafish, the fertilized eggs of zebrafish were collected and divided into three treatment groups, the control group (without adding bacteria), the low-concentration group (adding 104 cfu/ mL FR27) and the high-concentration group (adding 105 cfu/mL FR27), and the treatment lasted for three weeks. The hatching rate of zebrafish eggs, the growth condition and development of juvenile fish were measured. The results showed that the addition of FR27 could increase the hatching rate of zebrafish eggs, the body length and fat deposition of juvenile fish, which were more significant (P<0.05) in the high-concentration group as compared with the other groups. The feed intake and movement of juvenile fish were increased in FR27-added group. In summary, we speculated that FR27 promotes juvenile fish growth and fat deposition by enhancing the feed intake and moving ability of zebrafish juveniles.

Key words: fermented bean curd, lactic acid bacteria, probiotics, zebrafish, growth promoting