生物学杂志

生物学杂志 ›› 2019, Vol. 36 ›› Issue (5): 16-.doi: 10.3969/j.issn.2095-1736.2019.05.016

• 研究报告 • 上一篇    下一篇

低温驯化对斑马鱼胚胎发育和mtDNA拷贝数的影响

  

  1. 1. 海洋生物科学国际联合研究中心（上海海洋大学） 中国科学技术部, 上海 201306; 
    2. 水产种质资源发掘与利用教育部重点实验室（上海海洋大学）, 上海 201306; 
    3. 水产科学国家级实验教学示范中心（上海海洋大学）, 上海 201306
  • 出版日期:2019-10-18 发布日期:2019-10-11
  • 通讯作者: 陈良标，教授，主要从事动物进化与环境基因组学研究，E-mail: lbchen@shou.edu.cn
  • 作者简介:冉皓宇，硕士研究生，研究方向为发育分子生物学，E-mail：rhyme0411@163.com
  • 基金资助:
    国家自然科学基金面上项目(31572611)；上海市教委水产高峰学科项目

Effects of cold acclimation on the embryogenesis and mtDNA copy number in zebrafish embryos

  1. 1. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306; 2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources， Ministry of Education, Shanghai Ocean University, Shanghai 201306; 3. International Research Center for Marine Bioscience (Ministry of Science and Technology), Shanghai Ocean University, Shanghai 201306, China
  • Online:2019-10-18 Published:2019-10-11

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摘要： 旨在探索低温驯化对斑马鱼胚胎线粒体DNA（mtDNA）拷贝数及胚胎低温发育能力的影响。27 ℃发育至性成熟的斑马鱼，在22 ℃驯化6个月，构建低温驯化模型。驯化组与未驯化组分别交配产卵后，收集0 hpf胚胎，在22 ℃和20 ℃下发育并观察胚胎的低温发育能力。提取0 hpf以及8 hpf、12 hpf、24 hpf 4个时期的胚胎DNA，通过绝对荧光定量PCR比较分析胚胎中mtDNA拷贝数的变化情况。结果显示，低温驯化后斑马鱼0 hpf胚胎mtDNA拷贝数显著低于未驯化组胚胎mtDNA拷贝数，且mtDNA拷贝数在胚胎发育后期的3个不同时期仍低于未驯化组胚胎，但差异逐渐缩小。同时，相比于未驯化胚胎，低温驯化后的斑马鱼胚胎在22 ℃低温具有更高的成活率，且可以在20 ℃低温胁迫下成活。研究表明，低温驯化致使斑马鱼胚胎mtDNA拷贝数降低，同时提高了斑马鱼胚胎的低温发育能力。

关键词: font-size:medium, ">低温驯化；斑马鱼；mtDNA；胚胎发育

Abstract: The purpose of this study was to explore the effect of cold acclimation on mitochondrial DNA (mtDNA) copies number and development ability at low temperature of zebrafish embryos. The zebrafish was raised under 27℃ until adulthood, and acclimatized under 22℃ for 6 months. Fertilized embryos of acclimation group and wild-type group zebrafishes were collected after mating, then observed the embryogenesis of embryos while developing under lower temperatures which were 22℃ and 20℃ from 0 hpf stage. DNA of the 0 hpf, 8, 12 and 24 hpf embryos was extracted, detected and analyzed comparatively by Absolute Quantification PCR. The results showed that in 0 hpf, the mtDNA copies number of cold acclimation group was significantly lower than that of 27℃ non-acclimation group, and kept the same trend in the later 3 stages, but the gap was narrowing. Meanwhile, the embryos which endured cold acclimation at 22℃ showed higher survival rate than that at 27℃ non-acclimation group. And the embryos could survive under 20 ℃ cold stress. 

Key words: cold acclimation, zebrafish, mtDNA, embryogenesis

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