Loading...

Table of Content

    18 April 2026, Volume 43 Issue 2 Previous Issue   
    Application of analytical ultracentrifugation in the characterization and quality control of biopharmaceuticals
    YU Xiaojuan, CHU Wendan, CHANG Qing, WANG Zhaoxing, ZENG Chengshi, WANG Feiyu, YU Chuanfei, LI Wenqi
    2026, 43 (2):  1.  doi: 10.3969/j.issn.2095-1736.2026.02.001
    Abstract ( 28 )   Save
    Analytical ultracentrifugation (AUC) is a classical biophysical technique that enables precise analysis of the physicochemical properties of biomacromolecules in solution without the need for labeling. By monitoring the sedimentation behavior of molecules under a high centrifugal field, AUC provides rich information including molecular weight, sedimentation coefficient, purity, aggregation state, interactions, and particle density. This article systematically elaborated on the basic principles, instrument composition, fundamental operations, and data analysis methods of AUC. It highlighted the applications of AUC in quality assessment of biopharmaceuticals such as monoclonal antibodies, viral vectors, and nucleic acid drugs, including higher-order structure characterization, aggregation state and fragment analysis, determination of viral empty capsid ratio, and process consistency evaluation. Finally, it looked forward to the future development prospects of AUC in terms of automation, high-throughput capabilities, and applications in emergingtherapies.
    Related Articles | Metrics
    Codon bias of O-methyltransferase genes in Papaver somniferum L.
    SANG Luxi, ZHANG Wei, WU Yanran, LI Cui, WU Bo, ZHOU Jiayu, LIAO Hai
    2026, 43 (2):  10.  doi: 10.3969/j.issn.2095-1736.2026.02.010
    Abstract ( 20 )   Save
    In this study, Codon W and EMBOSS tools were adopted to determine relevant parameters, including relative synonymous codon usage (RSCU), effective number of codons (ENc), codon adaptation index (CAI), and base composition, of 68Papaver somniferumL. O-methyltransferase (PsOMT) genes. Furthermore, neutrality plot, ENc-plot, and PR2-plot were used to identify the predominant factor shaping codon usage patterns. The results showed weak codon usage bias, and the low expression level of PsOMT genes, with the average ENc values of 48.94 and average CAI values of 0.22. The average GC content of 0.40 exhibited a descending pattern of GC1s>GC2s>GC3s with an uneven distribution, suggesting preferential usage of Adenine (A) / Uracil (U)-ending codons, especially at the terminal codon. The results of neutrality plot, ENc-plot, and PR2-plot indicated that natural selection functioned as the predominant force shaping codon usage patterns. Among PsOMT genes, there were 17 optimal codons with A/U as the terminal codon, of which AGA was the most frequent. These findings provide insights for codon optimization of PsOMT genes, thereby facilitating the enhanced synthesis ofP. somniferumL. alkaloid through genetic engineering.
    Related Articles | Metrics
    oning and functional analysis of the phytoene synthase (PjPSY2)gene from the fruits of Panax japonicus
    JIN Rui, TANG Tingting, HUANG Xilun, LIANG E, ZHANG Lai
    2026, 43 (2):  17.  doi: 10.3969/j.issn.2095-1736.2026.02.017
    Abstract ( 12 )   Save
    In this study, the phytoene synthase gene (PSY,PjPSY2) from the fruits of thePanax japonicusand conducted bioinformatics analysis and functional validation were cloned. The results showed that the open reading frame of thePjPSY2gene was 945 bp, encoding a protein with typical phytoene synthase structural characteristics, and exhibiting high homology with other known plantPSYgenes. RT-PCR analysis showed that thePjPSY2gene had been integrated into the genome of wild-typeArabidopsis thalianaand was expressed at the transcriptional level at significantly higher levels than in wild-type plants. Analysis of five carotenoid components and their contents preliminarily confirmed that thePjPSY2gene affects fruit coloration inP. japonicusby regulating the biosynthesis of carotenoids. This study provides a foundation for a deeper understanding of the molecular characteristics and functions ofPjPSY2gene inP. japonicusfruits, and the results offer a scientific basis for the genetic improvement and breeding ofP. japonicus.
    Related Articles | Metrics
    Preliminary transcriptome analysis of Freesia hybrida seedlings under high temperature stress
    ZHANG Yan, LI Bo
    2026, 43 (2):  24.  doi: 10.3969/j.issn.2095-1736.2026.02.024
    Abstract ( 8 )   Save
    The aim of this study was to elucidate the molecular mechanisms ofFreesia hybridain responding to high-temperature stress and to promote the innovation of heat-tolerant germplasm,F. hybrida‘Red River’ was used as the experimental material. Physiological parameters were measured under heat stress at 40 ℃ for varying durations (3, 6, 9 and 12 hours). RNA-seq was conducted on seedlings exposed to 40 ℃ for 12 hours (stress group) compared to those maintained at 20 ℃ for 12 hours (control group). Differentially expressed genes (DEGs) were then identified and analyzed using GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment. Compared to that of the control group, a total of 2961 DEGs were identified after 12 hours of treatment at 40 ℃, including 1306 up-regulated and 1655 down-regulated genes. The GO analysis revealed that the significantly upregulated DEGs were primarily involved in response to heat, protein folding, protein complex oligomerization, response to reactive oxygen species (ROS), and protein import into the mitochondrial matrix. The KEGG pathway enrichment analysis identified several top-ranked metabolic pathways, such as protein processing in the endoplasmic reticulum, plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis, and photosynthesis-antenna proteins. Notably, the DEGs were consistent with the observed physiological changes under high-temperature stress. Specifically, 17 heat shock protein (Hsp) genes (out of 59 identified), 4 heat shock transcription factors (HSF) (out of 23), and the CUL3A gene exhibited significant up-regulation during thermal stress. Furthermore, transcription factors such as bZIP44, ERF63, ERF51, and NAC42 collectively formed a thermotolerance regulatory network inF. hybrida. Within the plant hormone signal transduction pathway, 18 DEGs were identified as being involved in the regulation of various phytohormones in response to high-temperature stress.
    Related Articles | Metrics
    Study on the biological decolorization mechanism of wild-type Shewanella oneidensis MR-1 in polyazo dye wastewater
    LIU Xuan, YANG Xingyu, YAO Xuan, ZHU Ling, AI Kaiyan, WU Chao
    2026, 43 (2):  32.  doi: 10.3969/j.issn.2095-1736.2026.02.032
    Abstract ( 14 )   Save
    To address the environmental pollution caused by azo dyes,Shewanella oneidensisMR-1 was selected as the model organism to study its capacity to reduce and decolorize the polyazo dye Direct Black 168 under anaerobic conditions, and to optimize degradation conditions. The results showed that the dye decolorization rate reached 96% when sodium lactate was used as the electron donor, with an incubation temperature of 30 ℃ and an initial inoculum of 5×107CFU/mL. Wild-typeS. oneidensisdecolorized polar polyazo dyes containing sulfonic acid groups via the Mtr pathway, whereas gene knockout strains exhibited inhibited dye degradation. Electron transporters enhanced dye decolorization. Within 2 h, the addition of 1 μmol/L riboflavin increased the decolorization efficiency from 33.1% to 70.2%. Electron acceptors competed with the DB168 for electrons, thereby inhibiting dye reduction. The addition of 20 mmol/L NaNO3and DMSO reduced decolorization rates to 49.5% and 6.6%, respectively. Through FTIR and LC-MS analysis, it was speculated that the dye molecule preferentially cleaved the azo bonds on both sides to generate aniline and 3,4-diaminophenol, both of which were readily oxidized. This study provides a theoretical basis for the application ofS. oneidensisin bioremediation.
    Related Articles | Metrics
    Study on the composition and diversity of the aquatic fungal in Namtso Lake in spring, Tibetan Plateau
    ZHENG Yanyan, HE Kai, WANG Lan, GUO Xiaofang, DE Ji
    2026, 43 (2):  39.  doi: 10.3969/j.issn.2095-1736.2026.02.039
    Abstract ( 8 )   Save
    This study investigated the fungal community structure and diversity in Namtso Lake during spring using high-throughput sequencing technology. The results showed that a total of 5791 OTUs were obtained from 20 sampling sites, which were classified into 17 phyla, 65 classes, 168 orders, 403 families and 869 genera by annotation. The dominant fungal communities were Ascomycota, Basidiomycota, and Mortierellaomycota. The α-diversity indices showed that the Shannon and Simpson diversity indices across the 20 sampling sites ranged from 2.01 to 7.10 and 0.39 to 0.98, respectively, while the Chao1 and ACE richness indices ranged from 672.51 to 1915.64 and 714.59 to 1920.35, respectively. PCoA and UPGMA cluster analyses indicated that the 20 sampling sites were grouped into four clusters, with the fungal community structures in the southwestern and northwestern regions exhibiting higher similarity. CCA results demonstrated that total dissolved solids (TDS), total phosphorus (TP), and turbidity were the key physicochemical factors significantly affecting the fungal community structure (P<0.05). The study explored the fungal community composition, diversity and their response to aquatic physicochemical factors in Namtso Lake, which provided a theoretical foundation for the utilization of fungal resources in lake and the protection of lake ecosystems in the Tibetan Plateau.
    Related Articles | Metrics
    Effects of cadmium stress on growth and metabolites of Parachlorella kessleri TY
    GAO Yifan, HE Meizhi, ZHU Lili, ZHENG Jinting, XIE Chengling, JI Li
    2026, 43 (2):  47.  doi: 10.3969/j.issn.2095-1736.2026.02.047
    Abstract ( 3 )   Save
    To investigate the physiological and biochemical responses ofParachlorella kessleriTY to cadmium stress, this study utilized a strain ofP. kessleriTY isolated from Shanxi Province.P. kessleriwere treated with cadmium solutions at mass concentrations of 0, 0.50, 1.50, 3.00, and 5.00 mg/L. Algal samples were collected daily during the stress period to measure growth, pigment content, chlorophyll fluorescence parameters, neutral lipid content, and total lipid content. The results showed that cadmium treatment inhibited the growth ofP. kessleriTY in a mass concentration-dependent manner, with inhibition rates reaching up to 62.9% at the highest mass concentration tested. Although chlorophyll content showed an increasing trend, it was still significantly inhibited (P<0.05). In the 3.00 mg/L treatment group, carotenoid content reached its highest level among all treatment groups (1.56 mg/L) but remained significantly lower than that of the control group. The chlorophyll fluorescence parameters (Fv/Fm,Fv/Fo, PIABS) of the algal cells decreased significantly with increasing cadmium mass concentration, indicating severely impaired photosynthetic capacity and PS Ⅱ photochemical activity. The protein content inP. kessleriTY exhibited a biphasic response to cadmium treatment, with inhibition at high mass concentrations and promotion at low concentrations. Furthermore, high mass concentrations of cadmium significantly promoted lipid accumulation in the microalgae, with the maximum increase reaching 169%. These findings revealed the toxic effects of cadmium onP. kessleriTY and provide a theoretical basis for its potential application in the treatment of low-concentration cadmium-containing wastewater.
    Related Articles | Metrics
    Effects of zinc and copper combined stress on growth and physiological indexes of alfalfa (Medicago sativa L.) seedlings
    SONG Xinmei, DUAN Shixin, WU Huaixin, LI Changyin, JIA Chunyun, LIU Changfeng
    2026, 43 (2):  54.  doi: 10.3969/j.issn.2095-1736.2026.02.054
    Abstract ( 4 )   Save
    Zinc (Zn) and copper (Cu) have been extensively used as feed additives. Owing to their low utilization efficiency by animals, substantial quantities of Zn and Cu were excreted in feces and subsequently entered the soil, leading to Zn and Cu pollution. This study used alfalfa (Medicago sativa L.) seeds as experimental material to examine the effects of combined Zn and Cu stress on various growth and physiological parameters of alfalfa seedlings, as well as their tolerance to Zn and Cu stress. Furthermore, correlation analysis and principal component analysis were conducted to provide a foundation for future research on the potential of alfalfa for remediating soil contaminated with Zn and Cu. The experiment was conducted using a hydroponic system, in which alfalfa seedlings were exposed to varying concentrations of Zn2+(0, 100, 200, 500 mg/L) and Cu2+(0, 50, 100, 200 mg/L) in a modified Hoagland solution. The findings indicated that the alfalfa seedlings accumulated Zn and Cu to some extent, with Zn accumulation being more pronounced under combined stress conditions. Correlation analysis demonstrated that, under the combined Zn and Cu stress, the concentrations of heavy metals, hydrogen peroxide (H2O2), and malondialdehyde (MDA) in the seedlings were positively correlated with the stress concentration. Conversely, the stress concentration was negatively correlated with both relative root cell viability and chlorophyll content. Principal component analysis identified hydrogen peroxide (H2O2), proline, and catalase (CAT) as pote
    Related Articles | Metrics
    Six species of the lichen genus Lecanora new to Anhui Province
    FENG Yishan, WU Xiaoying, LIANG Xiaoran, ZHANG Yanyun
    2026, 43 (2):  62.  doi: 10.3969/j.issn.2095-1736.2026.02.062
    Abstract ( 6 )   Save
    This study aimed to explore the species diversity and species identification characteristics of the genus Lecanora in Anhui Province. The taxonomic study based on phylogeny, morphology and chemistry for 208 Lecanora specimens collected from the province was carried out. Six species, Lecanora glabrata (Ach.) Nyl., L. symmicta (Ach.) Ach., L. fulvastra Kremp., L. pseudargentata Lumbsch, L. layana Lendemer and L. baekdudaeganensis B. G. Lee & Hur were newly recorded in Anhui Province. The detailed descriptions of the six species and a key to Lecanora in the province were provided. The results showed that the species diversity of Lecanora in Anhui Province is high, and this genus is one of the dominant genera in this region.
    Related Articles | Metrics
    The song response patterns of the Eurasian Nuthatch (Sitta europaea)to urban environments
    LIN Xiaogang, Shaliwa·Paiyizulamu, WU Qingming, ZOU Hongfei
    2026, 43 (2):  72.  doi: 10.3969/j.issn.2095-1736.2026.02.072
    Abstract ( 5 )   Save
    Urbanization has had a significant impact on urban wildlife, especially birds. As an essential behavioral adaptation to the environment, bird songs are highly sensitive to environmental changes. In order to explore the song response patterns of birds to urban development, the Eurasian Nuthatch (Sitta europaea), a typical resident bird in Heilongjiang Province was selected as the research subject. Songs data were collected from four urban parks in Harbin City and one remote nature reserve. The song sentence patterns and parameters (maximum frequency, minimum frequency, main frequency and duration) were analyzed using Avisoft-SASLab sound analysis software. A total of 14 song sentences were identified for the Eurasian Nuthatch. In the nature reserve, five distinct song sentences were recorded. However, the songs in urban parks were found to be relatively monotonous, with fewer sentences compared to those in the nature reserve. Significant variations were observed in song sentence diversity among different study sites. Regarding song parameters, the duration of songs in the nature reserve ranged from 0.35 to 3.124 s, exhibiting greater variability and diversity. In contrast, the duration of songs in urban parks exhibited a narrow range of variation, with significant differences among the various study sites. No significant differences were found in song frequency among the different study sites. These findings were of great significance for understanding the adaptive strategies of birds in response to urbanization and for the conservation of bird biodiversity. They also provide scientific evidence for the optimization and management of urban wildlife habitats.
    Related Articles | Metrics
    G1 ameliorates PTSD-like behaviors in mice by repairing damage to hippocampal neurons and astrocytes
    NIU Jingwen, YANG Shaojie, ZHU Guoqi, CHEN Lixia
    2026, 43 (2):  77.  doi: 10.3969/j.issn.2095-1736.2026.02.077
    Abstract ( 21 )   Save
    G1 is a specific agonist of G protein-coupled estrogen receptor 30 (GPR30). However, it remains unclear whether G1 can repair the damage to hippocampal neurons and astrocytes induced by post-traumatic stress disorder (PTSD). To investigate the effects of G1 on PTSD-like behaviors and its underlying mechanisms, a PTSD mouse model was established using the single prolonged stress (SPS) method and analyzed subsequent changes in hippocampal neurons and astrocytes. The results showed that mice in the SPS model group exhibited significant PTSD-like behaviors, including anxiety, depression, and impaired fear extinction (P<0.05). Concurrently, the expressions of GPR30, glial fibrillary acidic protein (GFAP), and connexin 43 (Cx43) in the hippocampus were downregulated. Following G1 intervention, PTSD-like behaviors were significantly ameliorated (P<0.05). The expressions of GPR30, GFAP, and Cx43 were upregulated, and the number of degenerated neurons in the hippocampus was notably reduced. These findings suggest that G1 improves PTSD-like behaviors in SPS mice, and its therapeutic mechanism may be associated with the repair of hippocampal neuronal and astrocyte damage.
    Related Articles | Metrics
    Research progress on the application of medicinal forage plant Agriophyllum squarrosum in antibiotic-free livestock farming
    QIAN Chaoju, YAN Xia, ZHANG Wanxiang, WANG Yan, MA Guorong, FAN Xingke, MA Xiaofei
    2026, 43 (2):  83.  doi: 10.3969/j.issn.2095-1736.2026.02.083
    Abstract ( 3 )   Save
    In the context of national “Big Food Concept” strategy and the antibiotic-free feed policies, this review systematically elucidates the research status and development potential of the annual desert medicinal plant sand rice (Agriophyllum squarrosum) as an antibiotic-alternative functional forage. Through a comprehensive review of recent research advances onA. squarrosum’s ecological adaptability, nutritional composition, pharmacologically active compounds, and feeding efficacy, sand rice is recognized as a pioneer desert species that demonstrates not only remarkable ecological benefits but also abundant nutritional and medicinal components, including proteins, vitamins, flavonoids, and alkaloids. These properties enable it to enhance livestock immunity, regulate gut microbiota, and support the transition toward antibiotic-free animal husbandry. Furthermore, sand rice exhibits multiple advantages for improving barren desert soils, alleviating forage shortages, and promoting modern grass-based livestock systems in desert regions of northwestern China. As a medicinal antibiotic-alternative forage, it has great potential for large-scale cultivation and application. However, there remains an urgent need to accelerate domestication and breeding programs for sand rice, which would help alleviate the shortage of high-quality forage, ensure the safety of livestock products in arid pastoral areas, and promote a balanced development of ecological security and the green economy in desert regions.
    Related Articles | Metrics
    Research progress on synthesis and application of extracellular polysaccharides from cyanobacteria
    LI Juan, XU Jie, LIU Xuefeng
    2026, 43 (2):  90.  doi: 10.3969/j.issn.2095-1736.2026.02.090
    Abstract ( 6 )   Save
    In this paper, the sugar-producing strains, synthesis pathways, influencing factors and applications of cyanobacterial extracellular polysaccharides were introduced. The four synthesis mechanisms of Wzx/Wzy-dependent pathway, ABC transporter-dependent pathway, synthase-dependent pathway and sucrase synthesis pathway were analyzed in detail, as well as the application prospects of cyanobacterial extracellular polysaccharides in the fields of medicine, food and environmental remediation. It pointed out that the current research on cyanobacterial exopolysaccharides has shortcomings in the analysis of the relationship between structure and function, the molecular regulation mechanism of the synthetic pathways, and large-scale production models. It proposed that the regulatory network of cyanobacterial exopolysaccharide synthesis should be further elucidated through multi-omics technology in the future, and efficient extraction and purification processes should be developed, and an optimization model of biomass and cyanobacterial exopolysaccharide production should be established to promote its in-depth development and industrial application in the fields of biomedicine, environmental remediation, and sustainable agriculture.
    Related Articles | Metrics
    Application of novel specific probes for mRNA capping rate detection
    WANG Zhenzhen, CHEN Ting, ZHANG Yunlong, LU Changrui, ZHANG Guilin
    2026, 43 (2):  97.  doi: 10.3969/j.issn.2095-1736.2026.02.097
    Abstract ( 4 )   Save
    The mRNA vaccine platform has emerged as a revolutionary technology in global public health and precision medicine, driven by its high immunoprotective efficacy, and flexible target design. As a critical quality attribute in industrial-scale mRNA production, capping efficiency directly impacts drug safety, efficacy, and manufacturing costs. However, current analytical methods suffer from limitations in accuracy, throughput, cost-effectiveness, and scalability. To address these challenges, this study innovatively optimized RNase H-based analytical strategies through rational probe design and streamlined purification processes. Seventeen sequence-specific probes with varied lengths and hybridization sites were systematically evaluated through RNase H digestion followed by LC-MS characterization. Three optimized probes demonstrated precise RNase H-mediated cleavage at the -2 position with over 99% efficiency. Subsequent silica-based purification effectively eliminated interference from uncut mRNA fragments, achieving consistent capping rates of 97% across multiple batches. This refined methodology establishes a robust, cost-effective platform for capping efficiency assessment, significantly advancing quality control paradigms for mRNA vaccine development.
    Related Articles | Metrics
    Optimization and practicing the teaching model for molecular biology experiments
    SHEN Hongyu, XIANG Benqiong, YIN Yanxia, LIU Yueqiao, CAO Yinghua
    2026, 43 (2):  103.  doi: 10.3969/j.issn.2095-1736.2026.02.103
    Abstract ( 9 )   Save
    In molecular biology laboratory instruction, students often struggle with an incomplete understanding of experimental procedures, inadequate pre-laboratory preparation, untimely feedback from instructors, and underdeveloped analytical skills. To address these challenges, this study reconfigured the laboratory curriculum. The curriculum focused on cloning the alkaline phosphatase gene and constructing prokaryotic expression strains. A project-based learning approach to enhance problem-solving skills were adopted using a blended learning model that integrated online and in-person instruction. Additionally, we prioritized enhanced communication and timely feedback, with particular emphasis on data integration and analysis. These revisions aimed to stimulate student engagement, improve instructional efficacy, and establish a solid foundation for training highly skilled, professional, and innovative educators in the field.
    Related Articles | Metrics
    Application and effect of PBL case teaching method in botany teaching
    LUO Huolin, WANG Chendan, LIAO Pengfei, YANG Boyun
    2026, 43 (2):  107.  doi: 10.3969/j.issn.2095-1736.2026.02.107
    Abstract ( 10 )   Save
    This study proposes a new botany teaching model that integrates problem-based learning (PBL) with the case teaching method. This model constructs a problem-centered case teaching framework, designed to guide students in applying botany knowledge to solve practical problems through teamwork and discussion. To achieve this goal, a diversified case system was designed, comprising “basic knowledge cases”, “production practice cases”, and “scientific frontier cases”. During implementation, careful attention was given to each aspect of the teaching model, including case selection, problem formulation, student grouping and discussion, and the presentation and evaluation of outcomes. The research results indicated that this PBL case teaching method not only significantly enhanced students’ mastery and application of botany knowledge but also comprehensively promoted the development of their overall abilities and qualities. Additionally, it effectively stimulated students’ interest and initiative in learning. This study extends the application of the PBL case teaching method, providing valuable guidance and a reference for the innovation of botany education.
    Related Articles | Metrics
    Construction and exploration of case database for graduate course of biochemical separation engineering
    YANG Lei, FAN Xiaoguang
    2026, 43 (2):  111.  doi: 10.3969/j.issn.2095-1736.2026.02.111
    Abstract ( 8 )   Save
    This paper systematically explored the entire process of constructing, applying, evaluating, and improving a teaching case database for the graduate course of biochemical separation engineering, with the aim of driving innovation and development of graduate education and teaching models through the establishment of such a case database. Initially, it clarified the purpose of establishing the case database, and elaborated on the critical steps from construction to screening of the case database, ensuring that the selected cases were both representative and of high practical value. Subsequently, it introduced the basic information of the current case database, along with its usage and management model, ensuring its efficient use in teaching practice. Regarding the application of the case database, it analyzed its positive impacts on student learning outcomes, teaching interaction patterns and other aspects, while revealing the effectiveness and potential of case-based teaching through an effectiveness evaluation. Finally, addressing the problems and challenges encountered during the implementation, we propose targeted improvement strategies and future development directions.
    Related Articles | Metrics
    Teaching reform and practice guided by enhancing student competencies of the first-class course microbiology
    ZHANG Aimei, ZHU Xuetai
    2026, 43 (2):  116.  doi: 10.3969/j.issn.2095-1736.2026.02.116
    Abstract ( 9 )   Save
    Leveraging the development of a first-class microbiology course as an opportunity, the teaching team formulated an overarching concept for innovative offline classroom teaching design, guided by the goal of enhancing student competencies. This concept focuses on the overall goal of the course, aims at the teaching pain points, promotes the innovation of teaching ideas, combines knowledge teaching with comprehensive quality training. The teaching innovation resulted in diverse exploratory and participatory teaching formats and models through measures such as the optimization of the teaching content system, the introduction of teaching cases and online platform resources, the application of multi-scene classroom teaching mode, participatory inverted classroom, interactive sharing activities and other classroom teaching measures. The teaching reform innovated the assessment and evaluation approach for the microbiology course throughout the entire learning process. After several years of teaching reform practice, the microbiology course innovations have proven to be highly effective in enhancing student competencies. Specifically, they have played a positive role in improving students’ abilities in language expression, PPT production, teamwork, innovative thinking, professional cognition, aesthetic creativity, practical operation, and professional learning. The teaching innovations have also yielded significant results in students’ performance in discipline competitions, postgraduate research selection, and vocational skills development.
    Related Articles | Metrics
    Innovative designs and practices in cellular morphology experiment teaching
    XIE Wenmei, LYU Yifei, WEI Jinping, CAO Yaning, LIU Liqiong
    2026, 43 (2):  121.  doi: 10.3969/j.issn.2095-1736.2026.02.121
    Abstract ( 4 )   Save
    In response to contemporary education’s evolving demands, this study took three cell morphology observation experiments as cases: cellular morphological structure, cell division, and human karyotype analysis, which innovatively changed from the conventional single-dimensional teaching model focusing on observation and verification in morphological experiments. Instead, it established a comprehensive design-based experimental teaching approach that employed “problem-based” guidance, “model-based” incentives, and “level-based” encouragement teaching strategies to enrich the diversity and depth of experimental teaching projects. The results showed that the implementation of exploratory comprehensive experimental teaching not only significantly improved the practicality and intuition of teaching, thereby enhancing students’ learning interest and effectiveness, but also effectively promoted the integration of multiple disciplines. This integrative approach allowed for the genuine synthesis of knowledge, stimulates critical thinking, and cultivated students’ innovative thinking and practical abilities. The study provided valuable insights and a reference for reforming cell morphology experiments teaching.
    Related Articles | Metrics
    Teaching research on molecular diagnosis and treatment technology of gastrointestinal tumors under the outcome-based education framework
    LI Na, CHEN Tingting, HUANG Qi, ZHANG Mingjun
    2026, 43 (2):  126.  doi: 10.3969/j.issn.2095-1736.2026.02.126
    Abstract ( 10 )   Save
    Molecular diagnosis and treatment technology for gastrointestinal tumors is a core tool in precision medicine; however, its clinical teaching faces issues such as vague objectives, overemphasis on theory over practical skills, and a single evaluation method, failing to meet the talent development requirements of the “New Medical Science” initiative. Centered on the concept of outcome-based education (OBE) and aligned with core clinical competency requirements, this study implemented teaching reforms from four dimensions: defining three-dimensional expected outcomes (knowledge, skills, and professional qualities), reverse-engineering a four-tier progressive teaching content system (“basic theory-clinical technology-case analysis-scientific innovation”), integrating six teaching models (including case-based teaching, virtual simulation, and multidisciplinary team (MDT) collaborative teaching), and establishing a closed-loop assessment system (“process evaluation (60%) + outcome evaluation (30%) + competence certification (10%)”). A total of 100 undergraduate medical students from the 2020 cohort were randomly divided into the study group (OBE teaching method) and the control group (traditional teaching method). The results showed that the theoretical score (89.64±12.54) and practical score (91.24±12.85) of the study group were significantly higher than those of the control group (84.26±12.12 and 63.58±8.88, respectively) (P<0.05). The total satisfaction score of the study group (90.34±12.15) was significantly higher than that of the control group (68.74±8.55) (P<0.01). Over 95% of students in the study group acknowledged the model’s effectiveness in improving autonomous learning, clinical thinking, and awareness of scientific innovation. This model facilitated the coordinated development of knowledge, skills, and professional qualities; provided a new paradigm for cultivating talents in gastrointestinal tumor molecular diagnosis and treatment in the context of “New Medical Science”; and offers a reference for clinical teaching reform in oncology.
    Related Articles | Metrics