Abstract: Twenty-one japonica (glutinous) rice varieties, which have been mainly planted in Anhui Province in recent years, were used as materials for polymorphism analysis according to the national standard (NY/T 1433—2014). Among the 48 SSR markers, 21 primers exhibited polymorphic between 21 materials, and a total of 59 alleles were detected, with an average of 2.81 per marker. The genetic diversity analysis showed that the variation of Shannon’s diversity index ranged from 0.199 to 1.163, with a mean value of 0.657; the variation of Nei’s index ranged from 0.095 to 0.620, with a mean value of 0.390. The polymorphic information content (PIC) ranged from 0.091 to 0.549,with a mean value of 0.331. There were some genetic differences among the 21 varieties.But, the observed number of alleles, Shannon’s index and Nei’s index, were low, indicating that the genetic background of the 21 varieties was narrow. The genetic similarity coefficients ranged from 0.426 to 0.954. The 21 varieties could be classified at 0.62 into five taxa. Based on the PIC and MI values of the markers, the polymorphic markers were further simplified and the standard fingerprint profiles of 21 japonica (glutinous) rice materials with 7 core SSR markers were constructed, which were prepared the ground for variety authenticity test, purity identification and core germplasm purification.

Key words: Japonica (glutinous) rice, genetic diversity, SSR marker, DNA fingerprinting