Abstract: In order to solve the problem in natural reproduction, domestication and transplanting of endangered plantTaihangia rupestrisvar.ciliate, its tissue culture technique was studied. Furthermore, based on the analysis of soil physicochemical properties in habitat ofT. rupestrisvar.ciliate,the soil conditions for domestication and transplanting of its cultured planets were established. The results were as follows: moderately mature leaves and petioles were the suitable explants for callus induction easy to be sampled with slight sampling disturbance to wildT. rupestrisvar.ciliate. The explants were sterilized in 0.1% aqueous mercuric chloride for 12 minutes, and then, had the lowest pollution rate and the highest success rate. The results of orthogonal test showed that the optimal medium formula for callus induction was as follows: 6-BA 0.1 mg/L+NAA 2.0 mg/L+2,4-D 0.5 mg/L ( or without 2,4-D )+agar 0.7 g/L+sucrose 30 g/L, pH 5.8. The problem of explant browning in callus induction ofT. rupestrisvar.ciliatewas effectively solved by reducing the culture temperature to 20 ℃. Then, the medium formula MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+agar 0.7 g/L+sucrose 30 g/L, pH 5.8, was the most appropriate for the differentiation of indefinite buds. And the rooting culture medium was 1/3 MS+IBA 0.5 mg/L+KT 0.15 mg/L+agar 0.7 g/L+sucrose 30 g/L, pH 5.8. Measurement results showed that the volume weight was 1.0－1.1 g/cm3, the pH was 7.7－7.8, and the total phosphorus (TP), organic matter and available manganese content of habitat soil were positively related to the growth ofT. rupestrisvar.ciliate, according to which the suitable transplanting medium for the cultured plantlets was garden soil∶rice husk carbon∶organic fertilizer 2∶2∶1 with relative air humidity 80%－90% and moderate shading in the early stage of transplanting. Under this cultivation technique, the survival rate reached 42.53%, and the growth was good.

Key words: Taihangia rupestrisvar.ciliata, tissue culture, habitat soil, physicochemical property, domestication and transplanting