Abstract: One rice variety，“Jingeng818”，was applied as material to screen glufosinate⁃tolerant（GT）rice mutants using heavy ion irradiation technique. Some physiological indicators of glufosinate tolerance were determined on the selected mutants and control rice groups after glufosinate spraying. The results showed that the leaf color of GT mutants maintained green with a significant higher chlorophyll content，while the leaves in control group were withered with lower chlorophyll content. After glufosinate spraying，the fresh biomass weight，dry weight and plant height of the GT rice mutants increased by 111.46%，53.40% and 48.00%，respectively，compared with those of the control group. The leaf ammonia content displayed the value lower than 0.8 mg/g in GT rice mutants，but the value was up to 1.3 mg/g in control. The glutamine synthase activities decreased both in the mutant rice and the control group，and there was no significant difference between the two treatments. Whether the glufosinate-tolerance mechanism of mutant rice is non-target resistance needs further verification.In conclusion，the screened GT rice mutants demonstrate a certain tolerance to glufosinate，and present huge potential in revealing molecular mechanism of glufosinate tolerance and variety breeding.

Key words: rice, glufosinate, chlorophyll, glutamine synthase, imazamox