Abstract: In order to investigate the effects of allyl isothiocyanate (AITC) on the synthesis of 5-HT in EC and the biological functions of EC, edible grade AITC were used to intervene with RIN-14B cells (rat pancreatic endocrine cell line), a cell model of EC. The calcium ion concentration in the cells was detected by FLUO-8 AM, the expression level of genes related to 5-HT synthesis was analyzed by qRT-PCR , the levels of 5-HT were determined by UPLC, and the transcriptome of AITC-treated RIN-14B cells was detected and analyzed by RNA-seq and bioinformatics.The results indicated that AITC caused an increase in intracellular calcium ion concentration through activation of TRPA1, while upregulated the expression ofTph1(tryptophan hydroxylase) and Ddc (5-hydroxytryptophan decarboxylase). In addition, GO and KEGG functional enrichment analysis on AITC treated RIN-14B cells showed that AITC mainly regulated glutathione metabolism and relate pathways such as antioxidant and inflammatory regulation, suggesting that AITC may regulate intestinal homeostasis by stimulating EC to promote glutathione metabolism, and also participate in inflammatory regulation of the intestine. The above results provided experimental data and research ideas for further study of the effects of AITC on EC and deeper biological functions of the intestine.

Key words: allyl isothiocyanate, enterochromaffin cells, 5-HT, RNA-seq, biological function