Abstract: A bacterium species was isolated from fecal specimens of white-headed langur. This bacterium is non-spore-forming, yellow pigment producing, gram-negative and catalase-positive. It was identified as Sphingobacterium daejeonense NS6-1 based on 16S rRNA gene sequence. NS6-1 can’t hydrolyze starch but is a heparin-degrading bacteria. The heparinase activity of NS6-1 was 46.60 U/mL, and can be increased to 107.29 U/mL in the present of 100 mmol/L Ca2+. Routine drug susceptibility test by K-B disk agar diffusion method showed that NS6-1 was resistant to kanamycin and gentamicin, while sensitive to rifampicin, tetracycline, polymyxin B, ofloxacin, cefoperazone and vancomycin. The genome was 4381042 bp in length with 37.21% overall GC content, including 3852 protein coding sequences. Functional annotation revealed a heparinase Ⅱ/Ⅲ-like protein in CAZy, a aminoglycoside resistance gene in CARD, a gene encoding catechol 1, 2-dioxygenas which can catalyze hydrocarbon-related catabolic in oil. Six gene clusters related to secondary metabolite biosynthesis were predicted by using antiSMASH, of which two were identified as carotenoid synthesis gene clusters and one emulsan synthesis gene clusters. In summary, NS6-1 is a heparin-degrading bacterium, with potential for degradation aromatic hydrocarbons in petroleum and carotenoid synthesis. The present results would lay a theoretical foundation for the further application of the isolated strain.

Key words: heparinase, Sphingobacterium, NS6-1, Trachypithecus leucocephalus