Journal of Biology ›› 2023, Vol. 40 ›› Issue (1): 40-.doi: 10.3969/j.issn.2095-1736.2023.01.040

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Impact of gene mutation of the rRNA methyltransferase LmrB on the lincomycin biosynthesis in Streptomyces lincolnensis #br#

XU Yurong1, XU Wanlian2, XU Jingjing3, ZHAO Ming1, GAO Liang2, WU Hang2   

  1. 1.Department of Chemical and Chemical Engineering, Hefei Normal University, Hefei 230601,
    China; 2.School of Life Sciences, Anhui University, Hefei 230601,China; 3.The First Affiliated Hospital of
    Anhui Provincial Hospital, Hefei 230001, China
  • Online:2023-02-18 Published:2023-02-21

Abstract: In order to explore the resistance function and the effect on lincomycin production of lmrB gene in Streptomyces lincolnensis NRRL2936 and LC-G,the lmrB gene from two different strains was ligated to different expression vectors pET28a and pIB139. The resistance test was carried out in E.coli BL21 and Streptomyces lividans TK24, respectively. lmrB gene in NRRL2936 and LC-G was respectively deleted by homologous recombination, and was over expressed in LC-G.The lincomycin production of S. lincolnensis and its derivatives was detected by HPLC. The results showed that although the length of LC-G-B gene fragment was shorter than that of 2936-B, LC-G-B did not lose its function, and the resistance of 2936-B to lincomycin was significantly higher than that of LC-G-B in E. coli and S.lividans. Meanwhile, the lincomycin yield of lmrB deletion mutant △lmrB was about 40% lower than that of NRRL2936. With the deletion of lmrB gene in LC-G, the yield of lincomycin was decreased by about 30%.The introduction of lmrB from NRRL 2936 into high-yield strain LC-G can significantly improve the yield of lincomycin.

Key words: Streptomyces lincolnensis, lincomycin, rRNA methyltransferase, resistance

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