Abstract: The different developmental stages fruits of Ziziphus jujuba Mill cv. Lingwuchangzao were used as experimental material to study the distribution of arabinogalactan proteins(AGPs) by βGlcY dyeing technology and confirmed by immunofluorescence localization. The main research results were followed: the brown-red precipitate formed by βGlcY-AGPs distributed in the cell walls and cell interiors of the epicarp and the alongside internal layers of closely arranged mesocarp small cells in different periods fruit. The brown-red precipitate formed by βGlcY-AGPs distributed in the cell walls and the inner part of the large ovoid parenchyma cells in the inner mesocarp during the early bulking period, while they were mainly distributed in the parenchyma cell walls during the other periods, with no distribution in the inner part of most cells. The brown-red precipitate formed by βGlcY-AGPs distributed in the cell walls and cell interiors of all cells in the vascular bundles in different periods fruit, the distribution of brown-red precipitate formed by βGlcY-AGPs gradually decreased in the mesocarp parenchyma cells with fruit development, this confirmed that AGPs gradually decreased. The intensity of brown-red precipitate formed by different concentration βGlcY was different at the same periods, the higher the concentration, the better the dyeing effect. There were some differences in brown-red precipitate intensity formed by the same concentration βGlcY in different tissues of fruit at different periods. βGlcY dyeing technology was effective method for AGPs histochemical localization of Ziziphus jujuba Mill cv. Lingwuchangzao fruit.

Key words: βGlcY dyeing technology, Ziziphus jujuba Mill cv. Lingwuchangzao, fruit, AGPs, histochemistry localization