生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (1): 17-.doi: 10.3969/j.issn.2095-1736.2026.01.017

• 研究报告 • 上一篇    下一篇

异养硝化—好氧反硝化假单胞菌TE-5脱氮条件优化

张宇红， 张　放， 王佳宝， 蔡佳诚， 姜月晴， 刘　健   

  1. 沈阳化工大学 环境与安全工程学院， 沈阳 110142
  • 出版日期:2026-02-18 发布日期:2026-02-27
  • 作者简介:张宇红，博士，副教授，研究方向为生物技术及污水处理，E-mail：zhangyuhong@syuct.edu.cn
  • 基金资助:
    辽宁省应用基础研究计划项目（2023JH2/101300047）；辽宁省教育厅面上项目（LJKZ0471）

Optimization of nitrogen removal conditions by heterotrophic nitrifying and aerobic denitrifying bacteria Pseudomonas sp. TE-5

ZHANG Yuhong, ZHANG Fang, WANG Jiabao, CAI Jiacheng, JIANG Yueqing, LIU Jian   

  1. College of Environmental and Safety Engineering, Shenyang University of Chemical Technology,
    Shenyang 110142, China
  • Online:2026-02-18 Published:2026-02-27

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摘要： 从某城市污水处理厂好氧池中分离筛选得到具有异养硝化和好氧反硝化（HN-AD）能力的Pseudomonassp. TE-5（TE-5）菌株。通过16S rDNA基因测序鉴定为假单胞菌属（Pseudomonassp.）。通过单因素实验初步确定菌株的较佳生长和硝化条件范围：柠檬酸钠作为最佳碳源，C/N 8~12，pH 6~9，摇床转速120~140 r/min，温度25~35 ℃，接种量2%~4%（体积比）。上述环境条件下，初始氨氮质量浓度100 mg/L，10 h脱氮率达到96%以上。结合Plackett-Burman design （PBD）试验、最陡上升路径法（SPAM）试验和响应面法（RSM）试验对菌株的多环境参数交互作用进行优化，确定菌株最优初始环境参数。RSM试验优化结果表明，初始氨氮质量浓度100 mg/L，7 h时，pH 9.08、摇床转速142.27 r/min、接种量3.56%（体积比）条件下，脱氮率达到90.96%±2.82%；10 h时脱氮率达到100%。结果表明，菌株TE-5具有良好的HN-AD性能，在废水生物处理中具有较高应用潜力。

关键词: 生物脱氮, 异养硝化, 好氧反硝化, Plackett-Burman设计, 响应面

Abstract: StrainPseudomonassp. TE-5 (TE-5), exhibiting both heterotrophic nitrification and aerobic denitrification (HN-AD) capabilities, was isolated and screened from the aerobic tank of a municipal wastewater treatment plant. Strain TE-5 was taxonomically identified asPseudomonassp. via 16S rDNA gene sequencing. Through single-factor experiments, the optimal range of growth and nitrification conditions for the strain was preliminarily determined. Sodium citrate was identified as the optimal carbon source. The cultural conditions were C/N 8－12, pH 6－9, speed 120－140 r/min, temperature 25－35 ℃, and inoculum dose 2%－4% (volume ratio). Under the above environmental conditions and with an initial ammonia nitrogen mass concentration of 100 mg/L, the nitrogen removal efficiency exceeded 96% within 10 h. The multi-environment parameter interactions of the strain were optimized by combining the Plackett-Burman design (PBD) experiment, the steepest ascent path method (SPAM) experiment, the response surface method (RSM) experiment, and the optimal initial environmental parameters of the strain were determined. RSM experiment optimization results indicated that, with an initial ammonia nitrogen concentration of 100 mg/L, the strain achieved a nitrogen removal efficiency of 90.96%±2.82% at 7 h under the following optimal conditions: pH 9.08, shaking speed 142.27 r/min, and inoculum dose 3.56% (volume ratio). After 10 hours, the nitrogen removal efficiency reaches 100%. The results indicate that strain TE-5 possesses good HN-AD performance and exhibits high application potential in wastewater biological treatment.

Key words: biological denitrification, heterotrophic nitrification, aerobic denitrification, Plackett-Burman design, response surface

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