生物学杂志

生物学杂志 ›› 2026, Vol. 43 ›› Issue (1): 26-.doi: 10.3969/j.issn.2095-1736.2026.01.026

• 研究报告 • 上一篇    下一篇

1 ,2-二氯乙烷降解菌YN5的筛选鉴定与土壤修复

杨宗政1,2,3， 王　浪1， 唐立娜1， 辛宗翰2， 吴　毅2， 吴志国1,2,3   

  1. 1. 天津科技大学 海洋与环境学院， 天津 300457； 2. 天津科技大学 化工与材料学院，
    天津 300457； 3. 天津市卤水化工与资源生态化利用重点实验室， 天津 300457
  • 出版日期:2026-02-18 发布日期:2026-02-27
  • 通讯作者: 吴志国，博士，副教授，研究方向为环境微生物及环境污染的生物修复，E-mail：wzhg@tust.edu.cn
  • 作者简介:杨宗政，博士，教授，研究方向为污染土壤修复，E-mail：yzz320@tust.edu.cn
  • 基金资助:
    VCM工厂含氯有机物污染土壤修复技术的研发及应用项目（2023120021000787）

Screening, identification, and soil remediation of a 1 ,2-dichloroethane degrading bacterium YN5

YANG Zongzheng1,2,3, WANG Lang1, TANG Li’na1, XIN Zonghan 2, WU Yi 2, WU Zhiguo1,2,3   

  1. 1. College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457,
    China; 2. College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology,
    Tianjin 300457, China; 3. Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilization,
    Tianjin 300457, China
  • Online:2026-02-18 Published:2026-02-27

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摘要： 从某化工厂的1,2-二氯乙烷（1,2-dichloroethane，1,2-DCA）污染土壤中筛选得到1株能够高效降解1,2-DCA的细菌YN5，通过形态观察、生理生化测试和16S rRNA序列分析，初步鉴定该菌株为屈曲杆菌属（Ancylobactersp.）。通过单因素实验考察菌株YN5的生长和降解特性，并将其应用于污染土壤修复中。实验结果表明，菌株YN5在pH 7~10、NaCl质量浓度1~10 g/L和温度20~40 ℃的条件下均能有效降解1,2-DCA。在最适条件（温度30 ℃、pH 9、NaCl质量浓度1 g/L）下，YN5可在16 h内将200 mg/L的1,2-DCA降解完全。在实验室模拟条件下，YN5对130 mg/kg 1,2-DCA污染土壤的2 d去除率达到77.10%。研究发现，土壤含水率和环境温度对菌株YN5的修复效果具有明显影响。此外，YN5修复后的土壤酶活性显著改善，其中对脲酶活性提升最为明显，可将脲酶活性提高130.83%。菌株YN5具有高效降解1,2-DCA的能力，在1,2-DCA污染土壤修复中展现出潜在的应用前景。

关键词: 1,2-二氯乙烷, Ancylobacter, 生物修复, 土壤修复, 土壤酶活

Abstract: In this paper, a bacterium YN5 capable of degrading 1,2-DCA was screened from soil contaminated with 1,2-dichloroethane (1,2-DCA) in a chemical plant. Through morphological observation, physiological and biochemical tests, and 16S rRNA sequence analysis, the strain was preliminarily identified asAncylobactersp.. The growth and degradation characteristics of strain YN5 were investigated by single factor experiment, and the strain was applied to the remediation of contaminated soil. The experimental results showed that strain YN5 could degrade 1,2-DCA effectively under the conditions of pH 7－10, NaCl mass concentration 1－10 g/L, and temperature 20－40 ℃. Under the optimal condition (temperature 30 ℃, pH 9, NaCl mass concentration 1 g/L), YN5 could completely degrade 200 mg/L of 1,2-DCA within 16 h. Under laboratory simulation conditions, the 2-day removal rate of YN5 on 130 mg/kg 1,2-DCA contaminated soil reached 77.10%. It was found that soil moisture content and ambient temperature significantly affected the remediation effect of strain YN5. In addition, the soil enzyme activity after YN5 restoration was significantly improved, with the urease activity showing the most obvious improvement, increasing by 130.83%. Strain YN5 had the ability to efficiently degrade 1,2-DCA efficiently, showing potential application prospects in the remediation of 1,2-DCA contaminated soil.

Key words: 1,2-dichloroethane, Ancylobacter, bioremediation, soil remediation, soil enzyme activity

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