生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (5): 34-.doi: 10.3969/j.issn.2095-1736.2024.05.034

• 研究报告 • 上一篇    下一篇

异养硝化-好氧反硝化菌ZH7的分离鉴定及脱氮特性研究

杨宗政1,2,3， 孟　珠1， 焦永杰4， 闫慧娟1， 左梓涵2， 张　晶1， 吴志国1,2,3   

  1. 1. 天津科技大学 化工与材料学院， 天津 300457； 2.天津科技大学 海洋与环境学院， 天津 300457；
    3.天津市卤水化工与资源生态化利用重点实验室， 天津 300457；
    4.天津市生态环境科学研究院， 天津 300074
  • 出版日期:2024-10-18 发布日期:2024-10-14
  • 通讯作者: 吴志国，博士，副教授，研究方向为环境微生物及环境污染的生物修复，E-mail:wzhg@tust.edu.cn
  • 作者简介:杨宗政，博士，教授，研究方向为生物强化技术在污水处理及其资源化方面的应用，E-mail:yzz320@tust.edu.cn
  • 基金资助:
    天津市科技计划项目（20YDTPJC01820）

Isolation, identification and nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification bacterium ZH7

YANG Zongzheng1,2,3, MENG Zhu1, JIAO Yongjie4, YAN Huijuan1, ZUO Zihan2,ZHANG Jing1, WU Zhiguo1,2,3   

  1. 1. College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology,
    Tianjin 300457, China; 2. College of Marine and Environmental Science, Tianjin University of Science & Technology,
    Tianjin 300457, China; 3. Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilization,
    Tianjin 300457, China; 4. Tianjin Academy of Eco-Environmental Sciences, Tianjin 300074, China
  • Online:2024-10-18 Published:2024-10-14

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摘要： 从养殖塘底泥中分离筛选出一株具有异养硝化-好氧反硝化功能的菌株ZH7，通过形态观察、生理生化测定和16S rDNA序列将菌株鉴定为不动杆菌属（Acinetbactersp.）。研究不同环境因素（碳源、碳氮比、温度、pH、摇床转速、NaCl浓度）对菌株生长及脱氮性能的影响，并在最佳条件下，探究菌株在单一氮源和混合氮源中的异养硝化-好氧反硝化性能。结果表明：ZH7可以乙酸钠为碳源，在C/N为12~16、温度30 ℃、摇床转速180 r/min、pH 7~9的条件下对氨氮（NH+4-N）和硝酸盐氮（NO－3-N）的去除率均可达到95%以上。混合氮源条件下（氨氮和硝酸盐氮），菌株会优先利用氨氮。氮源去除过程符合Compertz模型，菌株对氨氮的最大去除速率明显高于硝酸盐氮和亚硝酸盐氮，Rm分别为12.61、7.17和7.23 mg/(L·h)。菌株ZH7具有高效的脱氮能力，可作为生物脱氮候选菌株。

关键词: 不动杆菌, 异养硝化, 好氧反硝化, 生物脱氮, 含氮废水

Abstract: A heterotrophic nitrification-aerobic denitrification strain ZH7 was isolated from aquaculture pond sediment. The strain ZH7 was identified asAcinetobactersp. by morphological observations, physiological and biochemical assays and 16S rDNA sequencing. The impact of various factors (carbon source, C/N ratio, temperature, pH, shaking speed, NaCl concentration) on the strain’s growth and denitrification performance was investigated. And the heterotrophic nitrification and aerobic denitrification performance of the strain in single nitrogen source and mixed nitrogen source under the optimal conditions was also investigated. The results showed that the strain ZH7 could use sodium acetate as a carbon source, and achieved removal rates of ammonium nitrogen (NH+4-N) and nitrate nitrogen (NO－3-N) exceeding 95% under conditions of C/N of 12－16, temperature of 30 ℃, shaking speed of 180 r/min and pH value of 7－9. In mixed nitrogen source conditions (ammonium and nitrate nitrogen), the strain preferentially utilized ammonium nitrogen. The nitrogen removal process followed the Compertz model, with the maximum removal rate for ammonia nitrogen significantly higher than that for nitrate nitrogen and nitrite nitrogen, withRmof 12.61, 7.17 and 7.23 mg/(L·h), respectively. The strain ZH7 demonstrated the efficient denitrification capabilities and can be considered a potential candidate for biological denitrification.

Key words: Acinetbacter, heterotrophic nitrification, aerobic denitrification, biological nitrogen removal, nitrogenous wastewater

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