生物学杂志

生物学杂志 ›› 2024, Vol. 41 ›› Issue (5): 42-.doi: 10.3969/j.issn.2095-1736.2024.05.042

• 研究报告 • 上一篇    下一篇

一株异养硝化菌（Alcaligenessp. XN-1）的筛选分离及降解特性

武艳芳1,2， 甄　静1， 郭光光1,2， 梁欣冉1,2， 杜志敏3， 王继雯1， 李　磊1， 杨文玲1   

  1. 1. 河南省科学院生物研究所有限责任公司， 郑州 450008；
    2. 棕榈生态城镇发展股份有限公司， 郑州 450000； 3. 河南工业大学 环境工程学院， 郑州 450001
  • 出版日期:2024-10-18 发布日期:2024-10-14
  • 通讯作者: 甄静，副研究员，主要从事环境污染修复研究，E-mail:13629843529@163.com
  • 作者简介:武艳芳，硕士，高级工程师，主要从事微生物研究工作，E-mail:wuyanfang@palm-la.com
  • 基金资助:
    国家自然科学基金项目(32001206)； 河南省科学院项目（230605041， 210105002， 210405009）； 河南省重点研发专项项目（221111112300）； 河南省科技研发计划联合基金项目（225200810023）

Isolation and identification of a heterotrophic nitrification strain Alcaligenes sp. XN-1 and its degradation characteristics analysis

WU Yanfang1,2, ZHEN Jing1, GUO Guangguang1,2, LIANG Xinran1,2, DU Zhimin3,WANG Jiwen1, LI Lei1, YANG Wenling1   

  1. 1. Institute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou 450008, China;
    2. Palm Eco-Town Development Co., Ltd., Zhengzhou 450000, China;
    3. School of Environmental Engineering, Henan University of Technology, Zhengzhou 450001, China
  • Online:2024-10-18 Published:2024-10-14

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摘要： 为提高含盐废水的生物脱氮效率，从工业废水污泥中分离筛选出 1 株异养硝化细菌。对所分离的菌株进行形态学观察、革兰氏染色和分子生物学鉴定，16S rRNA基因测序结果在Blast 数据库中进行同源性分析并构建系统发育树，同时研究菌株在不同碳源、C/N、pH、温度和盐含量条件下的脱氮特性。结果表明：筛选得到的菌株鉴定为产碱杆菌属（Alcaligenessp.），命名为 XN-1；菌株XN-1在碳源为乙酸钠时具有最大的NH+4-N去除率和细胞生长量，其氨氮去除率和细胞生长量分别为100%和0.42；在C/N 15~20、pH 7~10、温度 30~35 ℃、盐含量0~4%条件下异养硝化效果最佳，氨氮去除率均为100%。菌株XN-1具有较高的异养硝化能力，在含盐废水脱氮处理中具有很大的应用潜力。

关键词: 异养硝化, 氨氮降解, 分离鉴定, 高盐

Abstract: To increase the biological nitrogen removal efficiency of these saline wastewater, a heterotrophic nitrification bacterium was enriched and isolated from industrial wastewater sludge. The strain was characterized by Gram staining and molecular biology. The 16S rRNA gene sequencing results were analyzed in Blast database for homology and a phylogenetic tree was constructed. The effects of carbon source type, different C/N, initial pH, temperature and NaCl concentration on the denitrification effect of the strain were studied. The results showed that the isolated heterotrophic nitrobacteria strain wasAlcaligenessp., named XN-1. Both the maximum NH+4-N removal rate and the maximum cell growth were obtained under sodium acetate with the values of 100% and 0.42, respectively When C/N 15－20, with initial pH 7－10, temperature at 30－35 ℃, and salinity in the rang of 0－4%, the highest ammonia nitrogen removal rate of 100% was obtained. Strain XN-1 had highly efficient heterotrophic nitrification ability and great potential in nitrogen removal treatment of saline wastewater.

Key words: heterotrophic nitrification, ammonia nitrogen degradation, isolation and identification, high salt

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