关键词: 川续断, 根蛋白, 响应面, 凝胶电泳

Abstract: In order to promote the proteomic investigation of Dipsacu asperides roots, it is necessary to analyze the protein extraction process and SDS-PAGE gel electrophoresis patterns of the roots. In this study, Tris-HCl method was used to extract the protein, and the differential bands of the root proteins from different producing areas were analyzed by SDS-PAGE gel electrophoresis. The extraction process was optimized by response surface. The results showed that the protein content of D. asperides roots in Hubei was the highest, followed by Guizhou and Yunnan. The gray value analysis of gel electrophoresis showed that two protein bands at 24 ku and 34 ku were significant differences in different habitats (Yunnan, Hubei and Guizhou) (P<0.05). Response surface methodology (RSM) was used to optimize the extraction process of D. asperides root protein, and the optimum extraction conditions were as follows: extraction temperature, 16.84 ℃; extraction time, 0.84 h; pH value, 6.58; solid-liquid ratio, 1∶ 1.64. The screened differential protein bands and the optimized extraction technology of D. asperides root protein can provide basic data for further understanding the molecular mechanism of the quality difference of D. asperides from different producing areas.

Key words: Dipsacu asperides, root protein, response surface, gel electrophoresis