生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 108-.doi: 10.3969/j.issn.2095-1736.2022.05.108

• 技术方法 • 上一篇    下一篇

响应面法优化南方红豆杉干细胞培养体系

  

  1. 江苏师范大学 生命科学学院 江苏省药用植物生物技术重点实验室, 徐州 221116
  • 出版日期:2022-10-18 发布日期:2022-10-19
  • 通讯作者: 曹小迎,博士,副教授,主要从事植物与微生物互作及植物分子机制研究,E-mail: cxy4868@jsnu.edu.cn
  • 作者简介:徐玲霞,硕士生,主要从事植物分子机制研究,E-mail: xulingxia96@163.com
  • 基金资助:
    国家自然科学基金项目(31770613); 江苏师范大学2020年度校级研究生科研创新计划项目(2020XKT494)

Optimization of cambial meristematic cells culture system of Taxus chinensis by response surface methodology #br# #br#

  1. Jiangsu Key Laboratory of Medicinal Plant Biotechnology, School of Life Sciences, Jiangsu Normal University,
    Xuzhou 221116, China
  • Online:2022-10-18 Published:2022-10-19

摘要: 红豆杉细胞培养是生产紫杉醇的有效途径之一,而干细胞的生长速度及紫杉醇的诱导生产要优于普通细胞。以南方红豆杉为材料,在单因素试验的基础上应用响应面法研究不同质量浓度的GA3、柠檬酸、抗坏血酸、活性炭对红豆杉干细胞的生长和褐化率的影响。响应面法分析表明:在干细胞基础培养基中,添加GA3 0.5 mg/L、柠檬酸150 mg/L、抗坏血酸100 mg/L和活性炭100 mg/L为促进南方红豆杉干细胞增殖、抑制褐化的最佳复配质量浓度。研究为南方红豆杉干细胞培养生产紫杉醇奠定基础。

关键词: 南方红豆杉, 干细胞培养, 培养基, 响应面分析

Abstract: Taxus cells culture is one of the effective ways to produce paclitaxel, and the growth rate and induction of paclitaxel production of cambial meristematic cells are better than that of the ordinary cells. The effects of different mass concentrations of GA3, citric acid, ascorbic acid and activated carbon on the growth and browning rate of Taxus chinensis cambial meristematic cells were studied by response surface methodology (RSM) on the basis of single factor experiment. Response surface methodology analysis showed that the optimal mass concentration of GA3 as 0.5 mg/L, citric acid as 150 mg/L, ascorbic acid as 100 mg/L and activated carbon as 100 mg/L in the cambial meristematic cells basal medium could promote the proliferation and inhibit the browning of Taxus chinensis cambial meristematic cells. This study laid the foundation for Taxus chinensis cambial meristematic cells culture to produce paclitaxel.

Key words: Taxus chinensis, cambial meristematic cell, culture medium, response surface analysis

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