生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (2): 75-.doi: 10.3969/j.issn.2095-1736.2023.02.075

• 研究报告 • 上一篇    下一篇

铁氧化菌促进水稻铁膜形成及其对Cd迁移的阻隔

陈金媛1,2， 靳梦楠1,2， 范芳娟1,2， 马绍晨1,2， 李苏骏1,2， 孙日洋1,2， 李　丁1,2   

  1. 1. 湖南工业大学 生命科学与化学学院， 株洲 412007；
    2. 百合种质资源创新与深加工湖南省工程研究中心， 株洲 412007
  • 出版日期:2023-04-18 发布日期:2023-04-18
  • 通讯作者: 李丁，博士，副教授，主要从事环境微生物的研究，E-mail: liding@hut.edu.cn
  • 作者简介:陈金媛，硕士在读，主要从事环境微生物的研究，E-mail: jinyuan0918@163.com
  • 基金资助:
    国家自然科学基金项目（32171622, 51774129）； 湖南省自然科学基金项目（2021JJ50003）； 湖南省教育厅研究项目（20B187）

Fe(II)-oxidizing bacterium promotes the formation of iron plaque and the prevention of Cd from soils to rice by iron plaque

CHEN Jinyuan1,2, JIN Mengnan1,2, FAN Fangjuan1,2, MA Shaochen1,2, LI Sujun1,2,SUN Riyang1,2, LI Ding1,2   

  1. 1.School of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China;
    2. Hunan Provincial Engineering Research Center of Lily Germplasm Resource Innovation and Deep Processing,
    Zhuzhou 412007, China
  • Online:2023-04-18 Published:2023-04-18

PDF (PC)

27

摘要： 通过硫化亚铁梯度管法和邻菲啰啉分光光度法，研究菌株LLDRA6的铁氧化特性。利用扫描电镜-能谱仪（scanning electron microscope-energy spectrum analysis, SEM-EDS）、连二亚硫酸钠－柠檬酸钠－碳酸氢钠（dithionite-citrate-bicarbonate, DCB）法，以及对两种盆栽水稻籽粒和茎叶的Cd含量检测，探讨菌株LLDRA6对IP形成的影响及其对水稻Cd迁移的阻隔效果。研究表明，LLDRA6在硫化亚铁梯度管中形成明显的Fe(II)氧化圈，接种该菌的LB培养基Fe(II)氧化率高于对照组，表明LLDRA6是一株铁氧化细菌。SEM-EDS显示，经LLDRA6和Fe(II)处理后的水稻根系表面附着有明显的层状沉淀物，沉淀物内含有大量Fe元素。DCB法提取试验显示：经LLDRA6和Fe(II)处理后的根系表面IP含量显著高于其他处理组，表明LLDRA6促进了IP的形成；该处理下的茎叶和籽粒的Cd含量均显著低于其他处理组。菌株LLDRA6促进水稻根系表面IP的形成，以及IP对Cd迁移具有显著的阻隔效果。

关键词: 铁氧化细菌, 铁膜, 水稻, 根系, 重金属, 镉

Abstract: Through the ferrous sulfide gradient tube and phenanthroline spectrophotometric experiments, the ability of Fe(II)oxidation for strain LLDRA6 was explored. By using methods such as scanning electron microscope-energy spectrum analysis (SEM-EDS), dithionite-citrate-bicarbonate (DCB) extraction test, and the measurement of Cd content in two rice varieties, the effect of strain LLDRA6 on the formation of IP and its effect on the migration of Cd from soils to ricewere discussed. The results showed that strain LLDRA6 formed an obvious Fe(II) oxidation circle in the ferrous sulfide gradient tube. In the presence of strain LLDRA6, the Fe(II) oxidation rate of LB liquid medium was higher than that of LB liquid medium without LLDRA6 (the control group), suggesting that this strain was an Fe(II)-oxidizing bacterium(FeOB); SEM-EDS results showed that, after addition of strain LLDRA6 and Fe(II) ions in soils, layered precipitates were formed on the surface of the rice roots, and the energy spectrum analysis (EDS) demonstrated that a large amount of Fe element were contained in these precipitates; the DCB extraction test showed that after treatment with strain LLDRA6 and Fe(II), the Fe content of IP was significantly higher than that of IP formed in other treatment groups, indicating that the strain facilitated the formation of IP. Moreover, the Cd content of the stems, leaves and grains were significantly lower than that of corresponding parts of rice grown in other treatment groups. These results demonstrated that strain LLDRA6 benefited to form IP around rice root surfaces, and the IP had a significant blocking effect on the migration of Cd from soils to rice.

Key words: Fe(II)-oxidizing bacterium, rice, iron plaque, roots, heavy metals, Cd

中图分类号: