生物学杂志

生物学杂志 ›› 2023, Vol. 40 ›› Issue (2): 65-.doi: 10.3969/j.issn.2095-1736.2023.02.065

• 研究报告 • 上一篇    下一篇

水稻抗倒伏和产量性状的相关性分析及QTLs定位

韩雷锋1, 周　燃1, 周　涛1, 林翠香2, 甘　泉2, 倪大虎2, 石英尧1, 宋丰顺2   

  1. 1. 安徽农业大学 农学院， 合肥 230036; 2. 安徽省农业科学院水稻研究所， 合肥 230031
  • 出版日期:2023-04-18 发布日期:2023-04-18
  • 通讯作者: 宋丰顺，博士，副研究员，研究方向为水稻分子育种，E-mail: sfs108@163.com
  • 作者简介:韩雷锋，硕士研究生，研究方向为水稻抗倒伏机理，E-mail: 1663729537@qq.com
  • 基金资助:
    安徽省科技重大专项(201903a06020012, 202003a06020005)； 安徽省自然科学基金项目(2008085MC98)

Correlation analysis and QTLs mapping of lodging resistance and yield traits in rice

HAN Leifeng1, ZHOU Ran1, ZHOU Tao1, LIN Cuixiang2, GAN Quan2,NI Dahu2, SHI Yingyao1, SONG Fengshun2   

  1. 1. College of Agronomy, Anhui Agricultural University, Hefei 230036, China;
    2. Rice Research Institute, Anhui Academy of Agricultural Science, Hefei 230031, China
  • Online:2023-04-18 Published:2023-04-18

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摘要： 以高产品种扬稻6号和抗倒伏品种绿旱1号构建的192个重组自交系为试验材料，对8个抗倒伏性状（株高、鲜重、抗折力、基部横切面积、基部髓腔面积、基部茎壁面积、壁厚和倒伏指数）和3个产量相关性状（穗长、单穗重、有效穗数）进行相关分析和QTL定位。共定位到抗倒伏相关QTL 14个，分布在第1、2、3、5、7、8、10、12号染色体上，LOD介于3.02~14.78，加性效应值在-5.22～2.36，QTL贡献率为3.36%～20%；产量相关QTL 8个，分布在第1、3、5、12 号染色体上，LOD介于3.1~9.43，加性效应值在-12.55～4.58，QTL贡献率为3.93%～14.53%。多个产量性状和抗倒伏性状之间呈显著负相关，且定位的多个QTLs呈簇出现。研究对抗倒伏性状和产量性状进行相关性分析和QTL定位，为分子设计高产、抗倒伏的优良水稻新品种提供参考。

关键词: 水稻, 抗倒伏, 产量, 关联分析, QTL定位； 连锁图谱

Abstract: With high-yield varieties Yangdao 6 and Lvhan 1 build 192 recombinant inbred lines as the experimental material, the eight lodging resistance traits (plant height, fresh weight, bending force, base transverse area, base area of the medullary cavity, basal stem wall area, wall thickness and lodging index) and three yield related traits (spike length, weight per panicle and effective panicle number) were selected for correlation analysis and QTL mapping. A total of 14 antiinversion related QTLS were identified, which were distributed on chromosome 1, 2, 3, 5, 7, 8, 10 and 12. LOD ranged from 3.02 to 14.78, additive effect ranged from -5.22 to 2.36, and QTL contribution rate was 3.36% to 20%. Eight yield related QTLS were distributed on chromosome 1, 3, 5 and 12, with LOD ranging from 3.1 to 9.43, additive effect value ranging from -12.55 to 4.58, and QTL contribution rate ranging from 3.93% to 14.53%. There was significant negative correlation between yield traits and lodging resistance, and many QTLs appeared in clusters. In this study, QTL mapping and correlation analysis were conducted for lodging resistance traits and yield traits, and the projection area method was adopted instead of wall thickness as an evaluation index to comprehensively excavate excellent QTL loci, providing scientific basis for breeding high yield and lodging resistance rice varieties.

Key words: rice, lodging resistance, yield, association analysis, QTL mapping, Linkage map

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