生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (5): 26-.doi: 10.3969/j.issn.2095-1736.2022.05.026

• 研究报告 • 上一篇    下一篇

裂殖酵母SpHMT1基因对拟南芥镉耐受性的影响

  

  1. 1. 广西大学 亚热带农业生物资源保护与利用国家重点实验室， 南宁 530004； 2. 广西大学 生命科学
    与技术学院， 南宁 530004； 3. 广西民族大学 海洋与生物技术学院， 南宁 530008
  • 出版日期:2022-10-18 发布日期:2022-10-18
  • 作者简介:黄婧，博士，助理研究员，研究方向为植物重金属修复、植物生长发育等，E-mail: jhuang@gxu.edu.cn
  • 基金资助:
    国家自然科学基金项目（31960660）； 广西自然科学基金项目（2017GXNSFAA198182， 2020GXNSFAA297211）

Role of Schizosaccharomyces pombe SpHMT1 gene in cadmium tolerance and accumulation in Arabidopsis thaliana

  1. 1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University,
    Nanning 530004, China; 2. College of Life Science and Technology, Guangxi University, Nanning 530004, China;
    3. School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, China
  • Online:2022-10-18 Published:2022-10-18

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摘要： 为分析裂殖酵母（Schizosaccharomyces pombe）重金属耐受因子基因SpHMT1（heavy metal tolerance factor 1）在拟南芥（Arabidopsis thaliana）镉耐受中的功能，通过在模式植物拟南芥中成功过量表达裂殖酵母跨液泡膜转运的PCs-Cd复合物重金属耐受因子基因SpHMT1，发现SpHMT1能增强植物对镉、砷、铜、锌等重金属的耐性和积累，并且对Cd2+的耐性和积累能力受到L-buthioninesulfoximine（BSO）的抑制。SpHMT1在拟南芥中定位于液泡膜上，能量发散X-射线微量分析（Energy-dispersive X-ray，EDX）发现转基因植物中硫元素（Sulfur，S）更多地存在于液泡中，说明SpHMT1在拟南芥中的功能依赖于植物螯合肽（Phytochelatins，PCs）。结果表明，SpHMT1延迟了重金属Cd2+从根到茎的长途转运，延缓了Cd2+在植株地上部分的积累。

关键词: SpHMT1基因, 拟南芥, 镉耐受性, 液泡, 植物螯合肽

Abstract: To study the function of Schizosaccharomyces pombe heavy metal tolerance factor 1 (SpHMT1) gene in Cd tolerance in Arabidopsis thaliana, the SpHMT1 gene encoding the PCs-Cd transporter in Arabidopsis was overexpressed. It was found that SpHMT1 enhanced the tolerance and accumulation of Cd, Cu, As and Zn, and failed to confer Cd2+ tolerance and accumulation when GSH and PC synthesis was blocked by L-buthioninesulfoximine (BSO). SpHMT1 was localized in tonoplast in Arabidopsis, and the energy-dispersive X-ray analysis showed that S was more present in vacuoles in transgenic plants, suggesting that the function of SpHMT1 in Arabidopsis depended on phytochelatins. On the other hand, constitutive expression of SpHMT1 delayed root to shoot transport of the metals, suggesting that roots can serve as a sink, with further reduction of metal content in shoots.

Key words: SpHMT1, Arabidopsis, cadmium tolerance, vacuole, phytochelatins

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