Abstract:

To provide a theoretical basis of a new rat model of glycation, the mice of glycation were induced by injecting D-galactose (200 mg kg-1 d-1) using injecting method for 45 days, which were measured the behavioral differences from model group to others and detected the index related to the glycation as AGEs, RAGE, sRAGE and esRAGE in variety organs of mice ( as brain, heart, liver, spleen, lung, kidney, skin, muscles and serum). The results showed that the DI of model group was decreased compared with that of the control group (P<0.01), though the number value of AGEs, RAGE, sRAGE and esRAGE of each tissues in mice were higher than that of control (P<0.01). This experiment investigated that it could make organism in a state of high glycosylation when they were injected by D-galactose (200 mg kg-1 d-1) for 45 days. The model could be used in the study of the etiopathogenesis of AGEs which could discover the receptors and the pathway of pharmaceutical drug. And the index changing form in multiple tissues would give the researchers some suggestions in experimental targets using the model.

Key words: D-galactose, glycation model, AGEs, RAGE, sRAGE, esRAGE