Abstract: Bovine type Ⅰ collagen was used as the research object. The main purpose was to develop the reference material of bovine type Ⅰ collagen. The characterizations, such as infrared spectroscopy (IR), triple helix structure, thermal stability, amino acid sequence coverage, terminal peptide residue, molecular weight et al, were analyzed. The homogeneity and stability were evaluated using the content of hydroxyproline and the SDS-PAGE, respectively. The value assignment and uncertainty assessment were evaluated using the content of hydroxyproline from eight laboratories. The results indicated that bovine type Ⅰ collagen met the requirement of collagen. The homogeneity of bovine type Ⅰ collagen was excellent in confidence coefficient of 95%. And it had good stability within 2 and 4 weeks at 4 ℃, 1 and 2 weeks at 25 ℃, repeated freezing and thawing 1, 3 and 5 times, and 6 months at －20 ℃. The certified value of the hydroxyproline was 11.17% with the expanded uncertainty of 0.03 in confidence coefficient of 95%. Therefore, the bovine type Ⅰ collagen could be used as national reference material for the quality evaluation of collagen products.