Abstract: In order to clarify the effect ofLycium barbarumexosomes on proliferation inhibition and apoptosis of non-small cell lung cancer cell A549, the exosomes were extracted by ultra-fast centrifugation method. The morphology of exosomes was detected by transmission electron microscopy, and the diameter distribution of exosomes was detected by particle size analysis. A549 cells were treated with the extractedL. barbarumexosomes. The effect ofL. barbarumexosomes on A549 cells proliferation was detected by CCK-8 method, EdU was used to detect the effect ofL. barbarumexosomes on A549 cells, the migration efficiency of cells was detected by cell scratch method and Transwell, the cloning ability of cells was detected by cell cloning method.The apoptosis rate of cells was detected by flow cytometry, Western Blot and qRT-PCR were used to detect the protein and gene expression of apoptosis pathway. The results showed that the extracted exosomes were saucer-like under electron microscope, and the size was 40－200 nm, which was consistent with the shape characteristics of the exosomes. Compared with control group,L. barbarumexosomes group could inhibit A549 cell proliferation in a concentration-dependent way (P<0.01). The cell migration ability and cell cloning ability in different concentrations ofL. barbarumberry exosomes decreased in a concentration-dependent manner (P<0.01), while the cell apoptosis rate increased in a concentration-dependent manner (P<0.01).L. barbarumexosomes could up-regulate the pro-apoptotic factors such as Cl-Caspase3, p53 and Bax in a concentration dependent manner, and down-regulate the proteins and genes of anti-apoptotic factors such as Bcl-2 (P<0.01). The conclusion was thatL. barbarumexosomes could inhibit the proliferation of A549 cells and promote the apoptosis of A549 cells, andL. barbarumexosomes could also induce apoptosis through mitochondrial pathway.

Key words: Lycium barbarum, exosome, non-small cell lung cancer cell, proliferation inhibition, apoptosis