Abstract: GPI(Glycosylphosphatidylinositol)-anchored proteins are complexes which consist of proteins, lipids and sugar chains. Studies indicate that GPI-anchored proteins are involved in various physiological processes such as immune recognition, cell communication and signal transduction. Exosomes regulate many pathophysiological processes including immune response and tumor growth, which make it significant to research the GPI-anchored proteins over the exosomes. In order to find the GPI-anchored proteins which can be used as the marker of the detection of lung cancer, 114 GPI-anchored proteins, 43 of which are significantly different between lung cancer patients and healthy people were studied. And the data were screened out using the mRNA expression array data of lung cancer patients and healthy people in TCGA (The Cancer Genome Atlas) database. DIA (Data Independent Acquisition) quantitative and Semi-quantitative technology were used to analysis of proteins from exosomes of NCI-H838 non-small cell lung cancer cell line, with total cellular membrane proteins as control, and 15 GPI-anchored proteins from exosomes were screened out. At last, 7 GPI-anchored proteins, including MSLN (mesothelin), CPM (carboxypeptidase M), SMPDL3B(sphingomyelin phosphodiesterase, acid-like 3B), HYAL2 (hyaluronoglucosaminidase 2), EFNA5(ephrin-A5), RECK(reversion-inducing-cysteine-rich protein with kazal motifs) and GPC1(glypican 1), were searched out by the data of GPI-anchored proteins in lung cancer patients and NCI-H838 cell exosomes, which can be used as the markers in the detection of lung cancer.

Key words: GPI-anchored proteins, exosome, the detection of tumor, marker