Abstract: The rhizosphere is one of the areas with the most active microbial activities and the closest relationship with plant growth. It is important to study the effect of plants on microorganisms to reveal the obstacles of continuous cropping. The fungal communities inrhizosphere soil ofPotentillaanserinaL. under different cropping years and different growth stages were investigated using the next-gen-eration sequencing. Results showed that fungal community covered 64 genera, 42 families, 31 orders,14 classes and 5 phylum, anddominated by five known fungal phyla, namely Ascomycota, Basidiomycota, Chytridiomycota, Glomeromycota and Zygomycota. Within the five phyla, it was found thatPhoma,Preussia,Cadophora,FusariumandMortierellawith average relative abundance were more than 1%. The samples from blooming stage accounted for the lowest diversity and they were significantly different from other growth sta-ges (P< 0. 05), and the samples from continuous cropping for 4 years were significantly higher than that of other continuous croppingyears (P< 0. 05). Soil nutrients had a greater impact on the structure of the fungal community, of which available potassium had the greatest impact (P<0. 05). The continuous cropping and growth ofPotentillaanserinaL. had significant influence on the structure ofrhizosphere fungal community, and maintaining the stability of the fungal community might be one of the ways for the high quality andefficient production ofPotentillaanserinaL.

Key words: PotentillaanserinaL. , continuous cropping, growth stage, rhizosphere, fungal community