Abstract: To investigate the hypermethylation status of microRNA-193b and microRNA-141 in septic shock mice, with the use of a mouse septic model induced by cecal ligation and puncture, the cardiac function was further detected with a multi-channel physiological signal acquisition system. The methylation statuses of both microRNA-193b and microRNA-141 in heart, liver, lung and small intestine tissues were detected by using bisulfite sequencing PCR and a real-time PCR method, respectively. The results indicated that the cardiac function was significantly impaired in the mice with late stage of sepsis shock. The methylation rate of microRNA-193b in heart tissue of the septic mice was 23.9%, 1.9% higher than that in normal tissue（P<0.05）. The methylation rates of microRNA-141 in lung and intestine tissue of the septic mice were 31.2% and 36.9%, 3.4% and 4.6% higher than those in normal tissue（P<0.01）, respectively. CpG islands in microRNA-193b and microRNA-141 gene promoter region were hypermethylated, which might be the reason for the changes in the expression of microRNA-193b and microRNA-141, suggesting that both of them might participate in the regulation on the pathophysiological state by regulating the protein expression of their downstream inflammation-related factors. Both microRNA-193b and microRNA-141 might be new biomarkers and targets for the infectious diseases diagnose and therapy.

Key words: sepsis, microRNA-193b, microRNA-141； DNA methylation