生物学杂志

Journal of Biology ›› 2025, Vol. 42 ›› Issue (4): 45-.doi: 10.3969/j.issn.2095-1736.2025.04.045

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Effect of Mycoplasma ovipneumoniae-induced ROS mediated oxidative damage through MAPK/NLRP3 signaling in sheep bronchial epithelial cells

LUO Jia1,2, XU Zhaokun1,3, LI Ya’nan1   

  1. 1. Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the
    Western China, College of Life Science, Ningxia University, Yinchuan 750021, China; 2. Institute of Medical Sciences,
    General Hospital of Ningxia Medical University, Yinchuan 750004, China; 3. Ningxia Polytechnic University,
    Yinchuan 750021, China
  • Online:2025-08-18 Published:2025-08-13

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Abstract: In this study, the impacts ofMycoplasma ovipneumoniae-induced ROS on oxidative damage were analyzed in primary bronchial epithelial cells (Ovis aries, Ningxia Tan sheep ) which were used to obtain an air-liquid interface ( ALI ) epithelial culture model. The results indicated thatM. ovipneumoniae-induced ROS production led to the release of inflammatory factors and initiation of apoptosis through activating MAPK ( ERK, JNK and p38 MAPK ) signaling-mediated NLRP3 signaling pathways. Meanwhile, during the NLRP3 inflammasome assembly, the activated caspase-1 improved the efficiency of activation of MAPK and forming a positive feedback loop. These data suggested thatM. ovipneumoniae-induced ROS production played an important regulatory role in the cellular pathogenesis ofM. ovipneumoniae-infected sheep bronchial epithelial cells through MAPK/NLRP3 signaling.

Key words: Mycoplasma ovipneumoniae, bronchial epithelial cells, air-liquid interface, NLRP3, oxidative damage

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