生物学杂志

Journal of Biology ›› 2025, Vol. 42 ›› Issue (1): 61-.doi: 10.3969/j.issn.2095-1736.2025.01.061

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Study on the inhibitory effect of crude extract of Agriophyllum squarrosum on the pathogen of blackshank disease of Zanthoxylum bungeanum in Longnan

SHI Liang1,2,3, QIAN Chaoju1,2, FAN Xingke1,2, YAN Xia4, CAO Yonghong5,ZHANG Hong6, LU Xin6, MA Xiaofei1,2   

  1. 1. Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Northwest Institute of
    Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; 2. Key Laboratory of
    Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Northwest Institute of Eco-Environment
    and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; 3. University of Chinese Academy of Sciences,
    Beijing 100049, China; 4. Key Laboratory of Inland River Ecohydrology, Cold and Arid Regions Environmental and
    Engineering Research, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences,
    Lanzhou 730000, China; 5. Longnan Academy of Non-wood Forest, Longnan 742100, China; 6. Gansu Yulinlong
    Low Carbon Energy Saving Technology Co., Ltd., Lanzhou 730000, China
  • Online:2025-02-18 Published:2025-02-12

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Abstract: In order to clarify the pathogenic fungi, morphological and ITS sequencing methods were used to isolate and identify the pathogenic fungi of black shank disease onZanthoxylum bungeanumin Longnan area. 13 strains with different colony morphological characteristics were isolated and purified from the black shank disease lesions of multiple LongnanZ. bungeanumstrains. After host tieback and Koch’s rule verification, it was clarified for the first time that the causative agent ofZ. bungeanumblack shank in Longnan was a type ofAlternaria tenuissima. The inhibitory effect of the crude extract ofAgriophyllum squarrosumagainstA. tenuissimawas evaluated by gradient growth inhibition assay. It was found that the crude extract of 8 mg/mL ofA. squarrosumhad the best inhibitory effect onA. tenuissimaand the inhibition rate reached at 70.18%. The research case not only illustrated the complex and diverse mechanisms of the occurrence of black shank inZ. bungeanum, but also supported that the crude extract of the psammophila medicinal plantA. squarrosumhad broad prospects as a botanical fungicide in the biological control of major diseases of important economic forest crops, and it expanded the application direction ofA. squarrosumin China’s characteristic ecological agriculture.

Key words: crude extract ofAgriophyllum squarrosum, black shank disease ofZanthoxylum bungeanum, pathogen;Alternaria tenuissima, fungicides of plant origin

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