Abstract: The common animal tissue DNA extraction method has been modified in the genetics course at Nankai University. This novel method of extracting DNA from Drosophila melanogaster can be applied in scientific research as well as in experimental teaching scenarios. The method comprises three steps which are namely alkali cracking, acid neutralization, and centrifugation. The method is characteristically simple, quick, stable, and cost-effective. The concentration and purity of the extracted DNA enable subsequent PCR amplification and are also conducive to experimental teaching. This method has simplified the process of extracting DNA from Drosophila melanogaster and has laid a foundation for the improvement of experimental projects related to Drosophila melanogaster gene expression analysis. In addition, it has helped to improve the experimental teaching effect and curriculum design.

Key words: Drosophila melanogaster, DNA extraction, alkaline lysis, genetic experiment, experimental teaching