Abstract: n order to study the secondary metabolites of Streptomyces sp. LRE-541 from the roots of Lilium davidii var. unicolor Cotton and its antigastric cancer activity, high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and other methods were used to isolate, purify and identify the secondary metabolites. The effects of metabolites on cell proliferation, apoptosis and migration of gastric cancer cell HGC-803 were investigated. Two anthraquinones were identified as 4-deoxy-ε-pyrromycinone (1) and epsilon-pyrromycinone (2). The IC50 of compound 1 and compound 2 against gastric cancer cell of HGC-803 were (25.24±1.23) μg/mL and (17.29±2.28) μg/mL, respectively. Under the same treatment, compared with blank control group, the proliferation rate, apoptosis rate and scratch healing rate of cancer cells in 15 and 25 μg/mL of compound 1 and 10 and 20 μg/mL of compound 2 groups were significantly decreased, the differences were statistically significant (P<0.05 or P<0.01). These results indicated that the two compounds could inhibit the proliferation and migration of gastric cancer of HGC-803 cells and promote the apoptosis of gastric cancer cells, which might be potential as anti-gastric cancer drugs or precursors.

Key words: ndophytic actinomycetes, anthraquinones, cell proliferation, apoptosis, cell migration