Abstract: In order to study the difference between the intersex and normal individuals, their testis and ovary were sequenced with Illumina Novaseq 6000 in this study, and were analyzed and compared in biological information and gene expression level. With sequencing, 83.79 Gb clean reads were obtained in total, and 6.29 Gb on average, the Q30 bases percentage was more than 94.25%. After assembling, 53077 Unigenes and 73588 transcripts were obtained, then they were mapped with the six international data, the mapping ratio was 18.17%-36.07%. The gene expression analyzing indicated that there were 3543 Unigenes co-expressed, the correlation of expression between ovaries was the greatest, the correlation for testis was followed, the correlation between ovary and testis was the last one. There were differences in the amount of expression gene between normal and intersex individuals, with 310 genes in total. In conclusion, besides differences in morphology between intersex and normal individuals, there were differences in transcription level for gonads, and the level was different for different kinds of intersex individuals.

Key words: red claw crayfish, intersex, transcription, characteristics, different analysis