Abstract: Chromosome fluorescence banding technology not only can reveal chromosomal basic features including size, primary constriction, secondary constriction, satellite etc., but also contributes to karyotpe analysis. Obviously, it plays a significant role in biological studies, such as plant evolutionary biology, chromosome research in human and animal, and so on. However, there has been no chromosome fluorescence banding related to biological experimental teaching for undergraduates and graduates in colleges at present. Therefore, for students experimental practice, we used Humulus lupulus, an economic crop, as material and designed a complete experimental teaching program, containing chromosome preparation and fluorescence microscopy technology. The experiment shows the chromosome fluorescence banding technology visually and systematically, and the result is meaningful with potential application value. Meanwhile, student can learn lots of experiment skills and develop their knowledge during this experimental practice. Besides, this experimental teaching program provides some beneficial suggestions for popularizing chromosome fluorescence banding technology in biological teaching course.

Key words: Humulus lupulus, sex chromosome, fluorescence banding, experimental course