Abstract: In order to accelerate the reproduction of Polygonatum sibiricum Hua, the paper established the tissue culture system of Polygonatum sibiricum Hua and studied the rapid propagation of tissue culture medium. The UPLC method was used for the determination of yam saponins and diosgenin in Polygonatum sibiricum Hua. The results showed that the optimal medium was MS+1.0 mg/L 6-BA+0.5 mg/L NAA+0.3 mg/L IAA. The optimal medium for hardening was peat soil and sand(2∶ 1). The total content of yam saponins and diosgenin was in the range of 90.49-174.42 μg/g.

Key words: Polygonatum sibiricum Hua, tissue culture, yam saponins, diosgenin, UPLC